Reagents and methods relating to DNA assays using amplicon probes on encoded particles
View Patent ↗Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead networking or other assay performance problems. Reagents for assaying DNA are described herein which include a plurality of encoded particles having attached amplicons amplified from a template DNA sequence. Each individual attached amplicon includes a nucleic acid sequence identical to a random portion of the template DNA sequence, wherein the amplicons together represent substantially the entire template DNA and wherein the nucleic acid sequence identical to a random portion of the template DNA sequence of each individual amplicon is shorter than the entire template DNA.
1. A reagent for assaying DNA, comprising:
a plurality of synthetic encoded particles having attached amplicons amplified from a template DNA sequence, wherein each individual synthetic encoded particle of the plurality of synthetic encoded particles comprises multiple different attached amplicons, wherein each individual attached amplicon comprises a DNA sequence identical to a random portion of the template DNA sequence, wherein the attached amplicons together represent substantially the entire template DNA and wherein the DNA sequence identical to a random portion of the template DNA sequence of each individual attached amplicon is shorter than the entire template DNA sequence.
2. The reagent of claim 1 , wherein the entire template DNA sequence has a length in the range of about 20-300 kilobases, inclusive and each individual attached amplicon comprises a DNA sequence having a length in the range of about 500-1200 nucleotides, inclusive, which is identical to a random portion of the template DNA sequence.
3. A multiplex reagent for assaying DNA, comprising:
a mixture of two or more pluralities of particles synthetic encoded such that synthetic particles of each plurality of synthetic particles are detectably distinguishable from synthetic particles of each other plurality of synthetic particles, the synthetic encoded particles having attached amplicons amplified from a template DNA sequence, wherein each individual synthetic encoded particle of the two or more pluralities of synthetic encoded particles comprises multiple different attached amplicons, wherein each plurality of synthetic encoded particles having attached amplicons amplified from a different template DNA sequence compared to each other plurality of synthetic encoded particles, each individual attached amplicon comprising a DNA sequence identical to a random portion of the template DNA sequence.
4. A kit for assaying DNA, comprising:
a mixture of two or more pluralities of particles synthetic encoded such that synthetic particles of each plurality of synthetic particles are detectably distinguishable from synthetic particles of each other plurality of synthetic particles, the synthetic encoded particles having attached amplicons amplified from a template DNA sequence, wherein each individual synthetic encoded particle of the two or more pluralities of synthetic encoded particles comprises multiple different attached amplicons, wherein each plurality of synthetic encoded particles having attached amplicons amplified from a different template DNA sequence compared to each other plurality of synthetic encoded particles, each individual attached amplicon comprising a DNA sequence identical to a random portion of the template DNA sequence.