Method for identifying olfactory receptor included in one olfactory cell

1. A method for identifying an olfactory receptor included in one olfactory cell among a mouse olfactory receptor group A; the method comprising steps of:

(a) obtaining the one olfactory cell;

(b) extracting mRNA from the one olfactory cell obtained in the step (a);

(c) obtaining cDNA with reverse transcriptase from the mRNA extracted in the step (b);

(d) amplifying the cDNA obtained in the step (c) by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02; wherein

SEQ ID:01 is A (adenine) -T (thymine) -G (guanine) -K (guanine or thymine) -C (cytosine) -I (inosine) -T (thymine) -W (adenine or thymine) -I (inosine) -G (guanine) -A (adenine) -Y (thymine or cytosine) -M (adenine or cytosine) -G (guanine) -I (inosine) -T (thymine) -A (adenine) -Y (thymine or cytosine) -G (guanine) -T (thymine) -I (inosine) -G (guanine) -C (cytosine); and

SEQ ID: 02 is T (thymine) -C (cytosine) -Y (thymine or cytosine) -T (thymine) -K (guanine or thymine) -R (guanine or adenine) -T (thymine) -T (thymine) -Y (thymine or cytosine) -C (cytosine) -T (thymine) -I (inosine) -A (adenine) -G (guanine) -R (guanine or adenine) -C (cytosine) -T (thymine) -R (guanine or adenine) -T (thymine) -A (adenine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -A (adenine) -G (guanine) -I (inosine) -G (guanine) -G (guanine) -R (guanine or adenine) -T (thymine) -T (thymine)

(e) determining whether or not a gene sequence of the cDNA amplified in the step (d) is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A;

(f) determining that, if the gene sequence of the cDNA is identical to the one gene sequence in the step (e), the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the step (e);

where the mouse olfactory receptor group A includes the following mouse olfactory receptors:

(Mouse olfactory receptor group A)

Olfr1, Olfr8, Olfr20, Olfr22-ps1, Olfr29-ps1, Olfr30, Olfr33, Olfr44, Olfr53, Olfr76, Olfr155, Olfr156, Olfr159, Olfr209, Olfr290, Olfr291, Olfr295, Olfr314, Olfr327-ps1, Olfr329, Olfr332, Olfr361, Olfr368, Olfr373, Olfr382, Olfr437, Olfr457, Olfr472, Olfr476, Olfr480, Olfr482, Olfr484, Olfr498, Olfr502, Olfr505-ps1, Olfr508, Olfr538, Olfr544, Olfr694, Olfr695, Olfr697, Olfr706, Olfr713, Olfr715, Olfr721-ps1, Olfr735, Olfr802, Olfr877, Olfr914, Olfr921, Olfr945, Olfr951, Olfr971, Olfr988, Olfr992, Olfr994, Olfr995, Olfr1032, Olfr1082, Olfr1084, Olfr1095, Olfr1097, Olfr1120, Olfr1145, Olfr1152, Olfr1162, Olfr1170, Olfr1173, Olfr1328, Olfr1425, Olfr1502 and Olfr1504.

2. The method according to claim 1 , further comprising:

disrupting a cell membrane of the one olfactory cell between the step (a) and the step (b).

3. The method according to claim 1 , wherein

the one olfactory cell is extracted from a sample solution containing olfactory cells in the step (a).

4. The method according to claim 1 , wherein

the mouse olfactory receptor group A is selected from the following mouse olfactory receptor group B:

(Mouse olfactory receptor group B)

Olfr 1, Olfr 8, Olfr 20, Olfr 22-ps1, Olfr 29-ps1, Olfr 33, Olfr 44, Olfr 76, Olfr156, Olfr209, Olfr290, Olfr291, Olfr295, Olfr314, Olfr327-ps1, Olfr329, Olfr332, Olfr361, Olfr368, Olfr437, Olfr472, Olfr476, Olfr480, Olfr482, Olfr484, Olfr502, Olfr505-ps1, Olfr508, Olfr538, Olfr694, Olfr695, Olfr697, Olfr706, Olfr721-ps1, Olfr735, Olfr802, Olfr877, Olfr914, Olfr921, Olfr945, Olfr951, Olfr971, Olfr988, Olfr992, Olfr994, Olfr995, Olfr1032, Olfr1082, Olfr1084, Olfr1145, Olfr1152, Olfr1170, Olfr1173, Olfr1502 and Olfr1504.