Microfluidic devices
View Patent ↗The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
1. A sample preparation method, the method comprising:
obtaining a plurality of unbound nucleic acids;
providing a plurality of aqueous droplets in a stream of oil flowing in a channel, wherein each aqueous droplet comprises a plurality of primer pairs in an aqueous solution completely surrounded by the oil;
introducing one or more of the unbound nucleic acids into each of the aqueous droplets; and
performing a sequencing reaction in each of the aqueous droplets,
wherein the unbound nucleic acids remain encapsulated in their respective aqueous droplets throughout the sequencing reaction.
2. The method according to claim 1 , further comprising conducting an amplification reaction in the aqueous droplets.
3. The method according to claim 2 , wherein the amplification reaction is selected from the group of PCR, QPCR, and rolling circle amplification.
4. The method according to claim 1 , wherein the oil comprises a surfactant.
5. The method according to claim 4 , wherein the surfactant is a fluorosurfactant.
6. The method according to claim 1 , wherein the unbound nucleic acids are obtained by shearing genomic DNA.
7. The method according to claim 1 further comprising:
hybridizing a plurality of the primer pairs to the nucleic acids; and
conducting a PCR reaction in at least one aqueous droplet.
8. A sample preparation method, the method comprising:
obtaining a plurality of unbound nucleic acids;
attaching a plurality of adapters to the unbound nucleic acids;
providing a plurality of aqueous droplets in a stream of oil flowing in a channel, wherein each aqueous droplet comprises a plurality of primer pairs in an aqueous solution completely surrounded by the oil;
introducing one or more of the unbound nucleic acids comprising the adapters into at least one of the aqueous droplets;
hybridizing one or more of the primer pairs to the unbound nucleic acids comprising the adapters in the at least one aqueous droplet; and
conducting a sequencing reaction in the at least one of the aqueous droplets,
wherein the unbound nucleic acids remain encapsulated in the aqueous droplets throughout the hybridizing and conducting steps.
9. A method for amplifying and sequencing a nucleic acid template, the method comprising:
attaching a plurality of adapters to an unbound nucleic acid template;
providing a plurality of aqueous droplets in a stream of oil in a channel, wherein at least one aqueous droplet comprises a plurality of primer pairs in an aqueous solution completely surrounded by the oil;
introducing the unbound nucleic acid template comprising the plurality of adapters into the at least one aqueous droplet;
hybridizing one or more of the primer pairs to the unbound nucleic acid template comprising the plurality of adapters in the at least one aqueous droplet;
amplifying the hybridized nucleic acid template comprising the plurality of adapters in the at least one aqueous droplet; and
performing a sequencing reaction in each of the aqueous droplets,
wherein the unbound nucleic acids remain encapsulated in the aqueous droplets throughout the hybridizing, amplifying, and performing steps.
10. The method according to claim 9 , wherein the oil comprises a surfactant.
11. The method according to claim 10 , wherein the surfactant is a fluorosurfactant.
12. The method according to claim 9 , wherein the amplification reaction is a PCR reaction.