Methods and compositions for delivering interleukin-1 receptor antagonist
View Patent ↗Methods, systems, and compositions related to generating and using a solution rich in interleukin-1 receptor antagonist are provided. Methods include contacting a liquid comprising white blood cells with a solid extraction material and stimulating with an electromagnetic field to activate production of interleukin-1 receptor antagonist. The interleukin-1 receptor antagonist can be separated from the solid extraction material. Methods for treating a site of inflammation in a patient include administering the solution rich in interleukin-1 receptor antagonist to the site of inflammation.
1. A method for generating a solution rich in interleukin-1 receptor antagonist comprising:
(a) contacting a liquid comprising white blood cells with a solid extraction material, wherein the solid extraction material activates the white blood cells to generate interleukin-1 receptor antagonist, and wherein the solid extraction material comprises a member selected from the group consisting of corundum, quartz, titanium, dextran, agarose, polystyrene, polyethylene, polyvinyl chloride, polypropylene, and combinations thereof;
(b) subjecting the liquid to an electromagnetic field wherein the electromagnetic field comprises a pulsed electromagnetic field or a capacitively coupled electromagnetic field; and
(c) separating the liquid from the solid extraction material to obtain a solution rich in interleukin-1 receptor antagonist, wherein the concentration of interleukin-1 receptor antagonist in the solution is greater than the concentration of interleukin-1 receptor antagonist in the liquid prior to contacting the liquid with the solid extraction material.
2. The method according to claim 1 , wherein the contacting the liquid comprising white blood cells with the solid extraction material and the subjecting the liquid to the electromagnetic field are performed in less than 1 hour.
3. The method according to claim 1 , wherein the liquid comprising white blood cells comprises whole blood, bone marrow aspirate, adipose tissue, fractions thereof, and mixtures thereof.