Treating bacteria with electric fields
View Patent ↗Cells that are in the process division are vulnerable to damage by AC electric fields that have specific frequency and field strength characteristics. The selective destruction of rapidly dividing cells can therefore be accomplished by imposing an AC electric field in a target region for extended periods of time at particular frequencies with particular filed strengths. Some of the cells that divide while the field is applied will be damaged, but the cells that do not divide will not be harmed. This selectively damages rapidly dividing cells like bacteria, but does not harm normal cells that are not dividing. Since the vulnerability of the dividing cells is strongly related to the alignment between the long axis of the dividing cells and the lines of force of the electric field, improved results can be obtained when the field is sequentially imposed in different directions.
1. A method of selectively destroying or inhibiting the growth of bacteria located within a target region, comprising the steps of:
capacitively coupling an AC electric field into the target region, wherein the frequency of the electric field is between 5 MHz and 20 MHz, wherein the strength of the electric field in at least a portion of the target region is between 2 V/cm and 100 V/cm, wherein the electric field has frequency characteristics that correspond to a vulnerability of the bacteria, wherein the electric field is strong enough to damage, during cell division, a significant portion of the bacteria whose long axis is generally aligned with the lines of force of the electric field, and wherein the electric field leaves non-dividing cells located within the target region substantially unharmed; and
repeating the coupling step until a therapeutically significant portion of the bacteria die.
2. The method of claim 1 , further comprising the step of delivering an antibiotic to the target region so that a therapeutically effective dose of the antibiotic is present in the target region while the coupling step is performed.
3. The method of claim 1 , wherein the strength of the electric field in at least a portion of the target region is between 5 V/cm and 20 V/cm.
4. The method of claim 1 , wherein the strength of the electric field in at least a portion of the target region is between 10 V/cm and 17 V/cm.
5. The method of claim 1 , wherein the frequency of the electric field is about 10 MHz.
6. The method of claim 1 , wherein the electric field has a first orientation during a first interval of time and a second orientation during a second interval of time, wherein at least a portion of the first interval of time and the second interval of time are mutually exclusive.
7. The method of claim 6 , wherein the first orientation is generally perpendicular to the second orientation.