Analyte sensor with increased reference capacity
Systems and methods of use for continuous analyte measurement of a host's vascular system are provided. In some embodiments, a continuous glucose measurement system includes an electrochemical sensor incorporating a silver/silver chloride reference electrode, wherein a capacity of the reference electrode is controlled.
1. An implantable electrochemical sensor for measuring an analyte concentration in a host, comprising:
a working electrode configured to measure a concentration of an analyte; and
a silver/silver chloride reference electrode, wherein at least a portion of the silver/silver chloride reference electrode is covered with an enzyme layer, wherein the enzyme layer is configured, in vivo, to generate hydrogen peroxide upon exposure to a substrate, whereby the hydrogen peroxide regenerates silver chloride of the reference electrode such that a reference capacity of the reference electrode is increased.
2. The implantable electrochemical sensor of claim 1 , wherein the enzyme is an oxidase enzyme.
3. The implantable electrochemical sensor of claim 2 , wherein the substrate is selected from the group consisting of glucose, urate, ascorbate, citrate, L-lactate, succinate, D-glucose, and ethanol.
4. The implantable electrochemical sensor of claim 1 , wherein the analyte is glucose.
5. The implantable electrochemical sensor of claim 1 , wherein the reference electrode comprises a chloridized elongated silver body.
6. The implantable electrochemical sensor of claim 1 , further comprising a diffusion barrier configured to substantially block diffusion of hydrogen peroxide between the silver/silver chloride reference electrode and the working electrode.
7. The implantable electrochemical sensor of claim 1 , wherein the enzyme layer has a thickness from about 0.01 microns to about 12 microns thick.
8. The implantable electrochemical sensor of claim 1 , wherein a percentage of a surface area of the reference electrode covered with the enzyme layer is from about 10% to about 100%.
9. An electrochemical sensor for measuring an analyte concentration in a host, comprising:
a working electrode configured to measure a concentration of an analyte; and
a reference electrode, wherein at least a portion of the reference electrode is covered with an enzyme layer, wherein the enzyme layer is configured, in vivo, to generate a reference electrode regenerating species upon exposure to a substrate, whereby the reference electrode regenerating species regenerates a component of the reference electrode such that a reference capacity of the reference electrode is increased.
10. The electrochemical sensor of claim 9 , wherein the enzyme is an oxidase enzyme.
11. The electrochemical sensor of claim 9 , wherein the substrate is selected from the group consisting of glucose, urate, ascorbate, citrate, L-lactate, succinate, D-glucose, and ethanol.
12. The electrochemical sensor of claim 9 , wherein the analyte is glucose.
13. The electrochemical sensor of claim 9 , wherein the reference electrode comprises a chloridized elongated silver body.
14. The electrochemical sensor of claim 9 , wherein the reference electrode regenerating species is hydrogen peroxide.
15. The electrochemical sensor of claim 9 , wherein the enzyme layer has a thickness from about 0.01 microns to about 12 microns thick.
16. The electrochemical sensor of claim 9 , wherein a percentage of a surface area of the reference electrode covered with the enzyme layer is from about 10% to about 100%.