Lateral flow assays
View Patent ↗Assays and methods including mobile tagged single stranded nucleic acid reagents pre-loaded on an analysis device, which are preferably tagged, but not labeled and are complementary to a strand (preferably the anti-sense strand in double stranded DNA targets) of the target nucleic acid. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. In addition, the analysis device includes a detection zone including one or more test zones that have an immobilized tag that binds to the tag on the mobile nucleic acid reagent.
1. A test strip for detection of at least one target nucleic acid in a sample comprising:
at least one detection zone comprising at least one test zone;
a sample application zone for applying the sample to the test strip;
at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acid sequences complementary to a first portion of a sequence of the target nucleic acid and at least one tag portion comprising a first tag, wherein the mobile tagged nucleic acid reagent does not include a detectable label and wherein the mobile tagged nucleic acid reagent is loaded in a reagent zone;
at least one second immobilized tag that binds to the first tag, wherein the immobilized tag is immobilized in a test zone on the test strip; and
a running buffer that includes at least one detectable label, wherein the detectable label is a dye that binds to double stranded nucleic acids; and
wherein the sample, the mobile tagged nucleic acid reagent, and the immobilized tag are all loaded on the test strip at locations such that the sample encounters the mobile tagged nucleic acid reagent and the immobilized tag while running an assay to detect the target nucleic acid in the sample.
2. The test strip of claim 1 , further comprising at least one lysis agent, wherein the lysis agent is loaded in a lysis zone located such that the lysis agent lyses the sample and a plurality of single strands of the target nucleic acid are accessible to the mobile tagged nucleic acid reagent at some point during the assay when the sample encounters the mobile tagged nucleic acid reagent.
3. The test strip of claim 2 , wherein the target nucleic acid is a double-stranded nucleic acid.
4. The test strip of claim 1 , wherein the target nucleic acid is a single-stranded nucleic acid.
5. The test strip of claim 1 , wherein the detection zone further comprises a control zone located beyond the test zone.
6. The test strip of claim 1 , wherein the detectable label is selected from the group consisting of a cyanine dye that nonspecifically binds to double stranded nucleic acids, a fluorescent dye that nonspecifically binds to double stranded nucleic acids and ethidium bromide.
7. The test strip of claim 1 , wherein the mobile tagged nucleic acid reagents comprises peptide nucleic acid (PNA) sequences.
8. The test strip of claim 1 , wherein the tag and the immobilized tag comprise a pair selected from the group consisting of:
a) a first biotin tag and a second immobilized tag selected from the group consisting of avidin, neutravidin and streptavidin;
b) a first tag selected from the group consisting of avidin, neutravidin and streptavidin and a second biotin immobilized tag;
c) a first lectin tag and a second glycosyl moiety immobilized tag; and
d) a first glyosyl moiety tag and a second lectin immobilized tag.
9. The test strip of claim 1 , further comprising a lysis agent or a denaturant.
10. The test strip of claim 1 , wherein the target nucleic acid is not amplified.
11. A test kit for detection of at least one target nucleic acid in a sample comprising:
a test strip comprising:
at least one detection zone comprising at least one test zone;
a sample application zone for applying the sample to the test strip;
at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acids complementary to a first portion of a sequence of the target nucleic acid and at least one tag portion but not including a label, wherein the mobile tagged nucleic acid reagent is loaded in a reagent zone;
at least one lysis agent loaded in a lysis zone wherein the lysis agent processes the sample such that the target nucleic acid can bind to the mobile tagged nucleic acid reagent when the target nucleic acid encounters the mobile tagged nucleic acid reagent;
at least one immobilized tag that binds to the tag portion of the mobile tagged nucleic acid reagent, wherein the immobilized tag is immobilized in a test zone of an assay; and
a running buffer that includes at least one detectable label, wherein the detectable label is a dye that binds to double stranded nucleic acids.
12. The test kit of claim 11 , wherein the sample, the mobile tagged nucleic acid reagent, the lysis agent and the immobilized tag are all loaded on the test strip at locations such that:
if the target nucleic acid is present in the sample, the target nucleic acid binds to the mobile tagged nucleic acid reagent to form a double stranded nucleic acid complex;
the double stranded nucleic acid complex binds to the dye in the running buffer to form a labeled duplex complex; and
the immobilized tag binds to the tag portion on the mobile tagged nucleic acid reagent of the labeled duplex complex while running an assay to detect the target nucleic acid in the sample.