Cow rumen xylose isomerases active in yeast cells
View Patent ↗Polypeptides were identified among translated coding sequences from a metagenomic cow rumen database, that were shown to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate.
1. A recombinant yeast cell comprising a heterologous nucleic acid molecule encoding a polypeptide having xylose isomerase activity and amino acid sequence with greater than 95% sequence identity to the amino acid sequence of SEQ ID NO:1, wherein the polypeptide provides xylose isomerase activity in the yeast cell.
2. The recombinant yeast cell of claim 1 wherein the amino acid sequence has at least about 95% identity to either of SEQ ID NO:1 or SEQ ID NO:3.
3. The recombinant yeast cell of claim 1 or 2 further comprising a complete xylose utilization pathway and having the ability to grow on xylose as a sole carbon source.
4. The recombinant yeast cell of claim 3 further comprising a target compound.
5. The recombinant yeast cell of claim 4 wherein the target compound is selected from the group consisting of ethanol, butanol, and 1,3-propanediol.