Bacterial xylose isomerases active in yeast cells
View Patent ↗Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate.
1. A recombinant yeast cell comprising a complete xylose utilization pathway including a codon optimized heterologous nucleic acid molecule encoding a polypeptide from a bacterium of the genus Lachnospiraceae selected from the group consisting of SEQ ID NOs:1, 3, and 5; wherein the codon optimization is for expression in a yeast cell, wherein the codon optimized heterologous nucleic acid molecule includes an operably linked promoter and is expressed producing xylose isomerase activity in the yeast cell; and wherein the heterologous nucleic acid molecule is chimeric.
2. The recombinant yeast cell of claim 1 further comprising the ability to grow on xylose as a sole carbon source.
3. The recombinant yeast cell of claim 2 further comprising a metabolic pathway that produces a target compound.
4. The recombinant yeast cell of claim 3 wherein the target compound is selected from the group consisting of ethanol, butanol, and 1,3-propanediol.
5. The recombinant yeast cell of claim 3 wherein the target compound is ethanol.