Method for testing treatment of ADAMTS13 deficiency
View Patent ↗The invention generally relates to methods of measuring cleaved von Willebrand factor (VWF) fragments. More specifically, the invention relates to methods of measuring the ability of a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) to cleave VWF in vivo. The invention also relates to methods of using various animal models which demonstrate ADAMTS13 activity similar to that of a human. The invention further relates to methods of measuring the cleavage products of rVWF in mammals, particularly in humans and in human plasma.
1. A method for testing effectiveness of a treatment for increasing a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) activity or concentration in a subject comprising measuring a level of von Willebrand Factor (VWF) cleavage fragments in a blood sample from the subject before and after the treatment, wherein an increase in VWF cleavage fragments or a decrease in the amount of ultra-large multimer forms of VWF with a reduced triplet structure after the treatment indicates that the treatment is effective in increasing ADAMTS 13 activity or concentration in the subject.
2. The method of claim 1 , wherein the subject suffers from von Willebrand disease (VWD) associated with a deficiency or dysfunction of ADAMTS13.
3. The method of claim 1 or 2 , wherein the treatment is administration of ADAMTS13 to the subject.
4. The method of claim 1 or 2 , wherein measuring the level of VWF cleavage fragments comprises performing Western blot analysis with a VWF antibody to visualize VWF cleavage fragments.
5. The method of claim 4 wherein the Western blot analysis is carried out under non-reducing conditions.
6. The method of claim 4 wherein VWF fragments are visualized through use of a VWF antibody conjugated to a marker.
7. The method of claim 6 wherein the marker is alkaline phosphatase (ALP) or horseradish peroxidase (HRP).
8. The method of claim 6 wherein the marker is detected with enhanced chemiluminescence (ECL).
9. The method of claim 4 wherein the VWF antibody is monoclonal or polyclonal.
10. The method of claim 1 or 2 , wherein VWF cleavage fragments are detected at a sensitivity level of about 0.025 to about 0.05 Ag U/mL VWF.
11. The method of claim 1 or 2 , wherein the blood sample is plasma or serum.
12. The method of claim 11 wherein the blood sample is plasma.
13. The method of claim 1 or 2 , wherein the subject is a mammal.
14. The method of claim 13 wherein the mammalian subject is human, rabbit, monkey, dog, rat, mouse, or pig.
15. The method of claim 14 wherein the subject is human, rabbit, monkey, or dog.
16. The method of claim 15 wherein the subject is human.
17. The method of claim 1 or 2 , wherein a change in the level of VWF cleavage fragments is detected by measuring the level of one or more of a 140 kDa VWF fragment or a 176 kDa VWF fragment.
18. The method of claim 17 wherein the VWF fragment is a 176 kDa VWF fragment.
19. The method of claim 1 , wherein the subject suffers from thrombotic thrombocytopenic purpura (TTP).
20. The method of claim 2 , wherein the treatment is administration of a von Willebrand Factor (VWF).
21. The method of claim 4 , wherein the VWF antibody is polyclonal.