Method for production of n-propanol and other C3-containing products from syngas using membrane supported bioreactor
This invention provides methods for the production of n-propanol. Specifically, the methods and systems of the present invention use symbiotic co-cultures operating in a membrane supported bioreactor for the production of n-propanol from syngas.
1 . A method for producing propanol and/or propionate comprising exposing gaseous substrates selected from the group consisting of carbon monoxide, carbon dioxide and hydrogen or combinations thereof to a symbiotic co-culture comprising a C1-fixing microorganism and a C3-producing microorganism both contained within biopores that open to a gas contact surface located on one side of an asymmetric hydrophilic membrane and circulating a fermentation liquid on a liquid contact surface located on the side of the membrane opposite to the gas contact surface to supply nutrients to the biopores and to recover propanol from the biopores while maintaining the symbiotic co-culture at conditions effective to convert the gaseous substrate into propanol and/or propionate.
2 . The method of claim 1 wherein a mixture of the C1-fixing microorganism and the C3-producing microorganism are introduced at the same time into the biopores of the asymmetric membrane.
3 . The method of claim 1 wherein the C1-fixing microorganism and the C3-producing microorganism are sequentially seeded into the biopores by first introducing a culture of the C3-producing microorganism into the biopores and subsequently introducing a culture of the C1-fixing microorganism into the biopores.
4 . The method of claim 1 wherein the C1-fixing microorganism and the C3-producing microorganism are sequentially seeded into the biopores by first introducing a culture of the C1-fixing microorganism into the biopores and subsequently introducing a culture of the C3-producing microorganism into the biopores.
5 . The method of claim 1 wherein the C1-fixing microorganism is a solventogenic homoacetogen, using the acetyl-CoA pathway.
6 . The method of claim 1 wherein the C1-fixing microorganism is selected from the group consisting of Clostridium Coskatii, Clostridium ljungdahlii, Clostridium authoethanogenium, Clostridium ragsdalei, Alkalibaculum bacchi, Clostridium thermoaceticum , and Clostridium aceticum.
7 . The method of claim 1 wherein the C3-producing microorganism is a propionogen.
8 . The method of claim 7 wherein the propionogen uses the lactate-acrylate pathway for the production of propionate.
9 . The method of claim 7 wherein the propionogen uses the methylmalonyl-succinate pathway for the production of propionate.
10 . The method of claim 1 wherein the C3-producing microorganism is selected from the group consisting of Clostridium neopropionicum, Clostridium propionicum, Pelobacter propinoicus, Desulfobulbus propionicus, Syntrophobacter wolinii, Syntrophobacter pfennigii, Syntrophobacter fumaroxidans, Syntrophobacter sulfatireducens, Smithella propionica, Desulfotomaculum thermobenzoicum subspecies thermosymbioticum, Pelotomaculum thermopropionicum , and Pelotomaculum schinkii.
11 . The method of claim 1 wherein the gaseous substrate is syngas.