Microfluidic device
Microfluidic devices of the present disclosure relate to quick and inexpensive microfluidic manipulation/handling. A number of channels may be supplied with fluid ingredient(s). In some embodiments, a number of protrusions as well as a sealing material may be disposed adjacent to the channels. When the channels are supplied with fluid ingredient(s), the channels may be partitioned into a number of separate cavities that are fluidly isolated from one another. For instance, a sealing material may be compressed so as to deform into the channels, obstructing fluid flow. In some embodiments, the channels supply fluid ingredients to a number of pre-formed cavities. Once the cavities are supplied with fluid ingredient, channels connecting the cavities may be sealed off; that is, the cavities may be subject to fluid isolation. When appropriate, contents within reaction chambers may be subject to further processing (e.g., thermal cycling, various analyses).
1. A microfluidic device, comprising:
a first layer defining a plurality of cavities;
a plurality of channels arranged to provide fluid entry to the plurality of cavities;
a second layer disposed adjacent to the first layer; and
wherein compression of the first and second layers relative to one another causes sealing of the plurality of channels resulting in obstruction of the plurality of cavities from further fluid entry and causes deformation of a sealing material into space defined by the plurality of channels, and wherein the first layer or the second layer comprises a material substantially more rigid than the sealing material.
2. The microfluidic device of claim 1 , wherein the first layer or the second layer comprises the sealing material.
3. The microfluidic device of claim 1 , wherein the sealing material is separate from the first layer and the second layer.
4. The microfluidic device of claim 3 , wherein the sealing material comprises the plurality of channels.
5. The microfluidic device of claim 1 , wherein compression of the first and second layers relative to one another causes plastic deformation of the sealing material.
6. The microfluidic device of claim 1 , wherein the sealing material includes an adhesive.
7. The microfluidic device of claim 1 , wherein the sealing material includes a wax.
8. The microfluidic device of claim 1 , further comprising a third layer disposed adjacent to the first layer and the second layer.
9. The microfluidic device of claim 8 , wherein the second layer or the third layer comprises an adhesive membrane disposed on a backing layer.
10. The microfluidic device of claim 1 , in combination with a system arranged to perform digital polymerase chain reaction.
11. The microfluidic device of claim 1 , wherein compression of the first and second layers relative to one another comprises irreversible sealing of the plurality of channels.
12. The microfluidic device of claim 1 , wherein the plurality of cavities are adapted to receive and isolate substantially equal volumes of a fluid ingredient.
13. The microfluidic device of claim 1 , wherein the plurality of cavities includes a first plurality of cavities and a second plurality of cavities disposed in fluid communication with one another, the second plurality of cavities adapted to retain a volume of air after filling of the first plurality of cavities.
14. The microfluidic device of claim 1 , wherein the first and second layers are adapted to be compressed by a roller.
15. The microfluidic device of claim 1 , wherein application of a single compressive force on the first and second layers relative to one another causes a fluid ingredient to be split into a plurality of substantially equal volumes fluidly isolated from one another.
16. The microfluidic device of claim 1 , wherein external compression of the first and second layers relative to one another causes sealing of the plurality of channels.
17. The microfluidic device of claim 1 , wherein the plurality of channels are substantially aligned with a plurality of protrusions.
18. The microfluidic device of claim 17 , wherein the second layer comprises the plurality of protrusions.
19. The microfluidic device of claim 1 , in combination with a system arranged to perform polymerase chain reaction.