Observation device, observation program, and observation system
View Patent ↗An observation device ( 1 ) is provided with: a general observation unit ( 10 ) for observing sample cells by observing an entire container (C) containing the cells and a culture solution; and a magnification observation unit ( 20 ) for magnifying a region within the container (C) and observing the cells, the general observation unit ( 10 ) and the magnification observation unit ( 20 ) each individually having lighting for illuminating the cells with light, and an optical system for observing the cells. The general observation unit ( 10 ) and the magnification observation unit ( 20 ) are thereby each provided with an individual optical system and lighting, making it possible to configure an appropriate observation unit for use both when the cells are observed by observing the entire container (C) and when a part within the container (C) is magnified and the cells are observed.
1. A non-transitory tangible machine-readable medium storing an observation program for causing a computer to execute processing, the processing comprising:
overall image sensing processing for taking an image of a sample through taking an image of an entire container containing the sample and a solution;
sample mass discrimination processing for discriminating from the image taken in the overall image sensing processing a sample mass, which is a mass of a plurality of pieces of the sample;
coordinate detection processing for detecting coordinates of a center of the sample mass discriminated in the sample mass discrimination processing;
magnified image sensing processing for taking an image of the sample mass on a magnified scale about, as a center, the coordinates detected in the coordinate detection processing; and
time keeping processing for counting days and hours from a start of observation of the sample, wherein
in the sample mass discrimination processing, discrimination of the magnified observation target sample mass is repeated every predetermined period counted in the time keeping processing with respect to sample mass discrimination until, from the image taken in the overall image sensing processing. any of sample masses, which are each a mass of a plurality of pieces of the sample, is recognized as a magnified observation target sample mass having a size equal to or larger than a predetermined size, and the discrimination of the magnified observation target sample mass is stopped on condition that a predetermined number of days have elapsed, and
in the coordinate detection processing, on condition that a magnified observation target sample mass having a size equal to or larger than the predetermined size has been recognized in the sample mass discrimination processing, coordinates of a center of the magnified observation target sample mass are detected.
2. The non-transitory tangible machine-readable medium according to claim 1 , wherein, in the coordinate detection processing, arbitrary coordinates are detected on condition that no magnified observation target sample mass has been recognized in the sample mass discrimination processing.
3. An observation system comprising:
an overall observation portion including
an illuminator which shines light on a sample inside a container containing the sample and a solution and
an optical system which guides light for observation of the sample,
the overall observation portion taking an image of the sample through taking an image of the entire container;
a time keeping portion which counts days and hours from a start of observation of the sample;
a data processing portion which repeats discrimination of a sample mass, which is a mass of a plurality of pieces of the sample, every predetermined period counted by the time keeping portion with respect to sample mass discrimination until a sample mass having a size equal to or larger than a predetermined size is recognized from the image taken by the overall observation portion, the data processing portion detecting coordinates of a center of the recognized sample mass having a size equal to or larger than the predetermined size;
a magnified observation portion including
an illuminator which shines light on the sample inside the container and
an optical system which guides light for observation of the sample,
the magnified observation portion taking an image of the sample mass on a magnified scale about, as a center, the coordinates detected by the data processing portion; and
a setting portion which sets
a sample mass discrimination repetition deadline until which the data processing portion recognizes the sample mass having a size equal to or larger than the predetermined size,
timing with which the magnified observation portion takes the image, and
an observation deadline until which the sample is observed.
4. The observation system according to claim 3 , wherein the data processing portion detects arbitrary coordinates on condition that no magnified observation target sample mass has been recognized.
5. A non-transitory tangible machine-readable medium storing an observation program for causing a computer to execute processing, the processing comprising:
overall image sensing processing for taking an image of a sample through taking of a container containing the sample and a solution:
sample mass discrimination processing for discriminating from the image taken in the overall image sensing processing a sample mass. which is a mass of a plurality of pieces of the sample;
coordinate detection processing for detecting coordinates of a center of the sample mass discriminated in the sample mass discrimination processing; and
magnified image sensing processing for taking an image of the sample mass on a magnified scale about, as a center, the coordinates detected in the coordinate detection processing, wherein
in the sample mass discrimination processing, when the discriminated sample mass has a figure with a predetermined roundness, or when a ratio of a major axis length to a minor axis length of an ellipse surrounding a contour of the discriminated sample mass is equal to or less than a predetermined threshold value, the discriminated sample mass is found to have a predetermined figure,
in the coordinate detection processing, on condition that the sample mass has been found to have the predetermined figure in the sample mass discrimination processing, the coordinates of the center of the sample mass are detected, and
in the coordinate detection processing, arbitrary coordinates are detected on condition that the sample mass has been found not to have the predetermined figure in the sample mass discrimination processing.
6. A non-transitory tangible machine-readable medium storing an observation program for causing a computer to execute processing, the processing comprising:
overall image sensing processing for taking an image of a sample through taking of a container containing the sample and a solution;
sample mass discrimination processing for discriminating from the image taken in the overall image sensing processing a sample mass, which is a mass of a plurality of pieces of the sample;
coordinate detection processing for detecting coordinates of a center of the sample mass discriminated in the sample mass discrimination processing;
magnified image sensing processing for taking an image of the sample mass on a magnified scale about, as a center, the coordinates detected in the coordinate detection processing; and
time keeping processing for counting days and hours from a start of observation of the sample, wherein
in the sample mass discrimination processing, when the discriminated sample mass has a figure with a predetermined roundness, or when a ratio of major axis length to a minor axis length of an ellipse surrounding a contour of the discriminated sample mass is equal to or less than a predetermined threshold value, the discriminated sample mass is found to have a predetermined figure,
in the coordinate detection processing, on condition that the sample mass has been found to have the predetermined figure in the sample mass discrimination processing, the coordinates of the center of the sample mass are detected, and
in the sample mass discrimination processing, the discrimination is repeated every predetermined period counted in the time keeping processing with respect to sample mass discrimination until the sample mass having the predetermined figure is recognized, and the discrimination is stopped on condition that a predetermined number of days have elapsed.
7. An observation system comprising:
an overall observation portion including
an illuminator which shines light on a sample inside a container containing the sample and a solution and
an optical system which guides light for observation of the sample,
the overall observation portion taking an image of the sample through taking an image of the entire container;
a time keeping portion which counts days and hours from a start of observation of the sample;
a data processing portion which repeats discrimination of a sample mass, which is a mass of a plurality of pieces of the sample, every predetermined period counted by the time keeping portion with respect to sample mass discrimination until a sample mass having a predetermined figure is recognized from the image taken by the overall observation portion, the data processing portion detecting coordinates of a center of the recognized sample mass having the predetermined figure;
a magnified observation portion including
an illuminator which shines light on the sample inside the container and
an optical system which guides light for observation of the sample,
the magnified observation portion taking an image of the sample mass on a magnified scale about, as a center, the coordinates detected by the data processing portion; and
a setting portion which sets
a sample mass discrimination repetition deadline until which the data processing portion recognizes the sample mass having the predetermined figure,
timing with which the magnified observation portion takes the image, and an observation deadline until which the sample is observed.