Anti-La antibodies and their use for immunotargeting
The invention relates to antibodies against the human La protein and to their use in immunotargeting, in particular the immunotargeting of tumor cells. The object of the invention is to provide improved antibodies which bind universal target structures on the surface of tumor cells, and to provide novel anti-La antibodies, in particular with a high affinity for La, a universal target structure on tumor cells, which make it possible to use the antibodies as recombinant fragments for immunotargeting. The invention comprises recombinant antibodies comprising: i. a binding unit of an antibody which specifically binds to an epitope of a human nuclear antigen, preferably human La protein, and ii. a binding unit of an antibody which specifically binds to an effector cell or of a ligand which specifically binds to an effector cell. The invention furthermore comprises novel antibodies which specifically bind the human La protein.
1. Recombinant antibody, comprising
a.) a binding unit of a first antibody that specifically binds to an epitope of a human nuclear antigen, wherein one such human nuclear antigen can comprise human La, characterized in that the first antibody is an antibody which binds specifically an epitope of the human La protein (anti-La antibody) containing regions determining complementarity (complementarity determining regions, CDRs), the CDRs of the variable region of the light chain (V L ) and the variable region of the heavy chain (V H ) comprising the following sequences:
SEQ
SEQ
ID
ID
V L
No.
V H
No.
CDR1
KSSQSLLNSRTPKNYLA
3
HYYIY
4
CDR2
WASTRKS
5
GVNPSNGGTHFNEKFKS
6
CDR3
KQSYNLLT
7
SEYDYGLGFAY
8
and
b.) a binding unit comprising
a second antibody which binds specifically to an effector cell or
a ligand which binds specifically to an effector cell.
2. The recombinant antibody according to claim 1 , characterized in that the second antibody or the ligand which binds specifically to an effector cell is selected from antibodies and ligands which bind specifically surface structures on T lymphocytes, NK cells and/or monocytes.
3. Antibodies which bind specifically an epitope of the human La protein (anti-La antibody) containing regions determining complementarity (complementarity determining regions, CDRs), characterized in that the CDRs of the variable region of the light chain (V L ) and the variable region of the heavy chain (V H ) comprise the following sequences:
SEQ
SEQ
ID
ID
V L
No.
V H
No.
CDR1
KSSQSLLNSRTPKNYLA
3
HYYIY
4
CDR2
WASTRKS
5
GVNPSNGGTHFNEKFKS
6
CDR3
KQSYNLLT
7
SEYDYGLGFAY
8.
4. The recombinant antibody according to claim 1 containing the following, optionally humanized, structure:
a variable region of the light chain having a sequence of SEQ ID No. 51 and
a variable region of the heavy chain having a sequence of SEQ ID No. 52.
5. The recombinant antibody according to claim 1 , in the form of a scFv fragment, a F(ab′) 2 fragment, diabodies, triabodies or tetrabodies.
6. The antibodies according to claim 3 , containing the following, optionally humanized, structure:
a variable region of the light chain having a sequence of SEQ ID No. 51 and
a variable region of the heavy chain having a sequence of SEQ ID No. 52.
7. The antibodies according to claim 3 in the form of a scFv fragment, a F(ab′) 2 fragment, diabodies, triabodies or tetrabodies.