Use of synergistic microorganisms and nutrients to produce signals that facilitate the germination and plant root colonization of mycorrhizal fungi in phosphorus rich environments
A composition of matter comprising: a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi that is placed in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root; and a method for increasing plant yield comprising: placing a combination of a phytate and a plurality of microorganisms comprising a Trichoderma virens fungus, a Bacillus amyloliquefaciens bacterium, and one or a plurality of mycorrhizae fungi in the vicinity of a plant root in a manner that allows the microorganisms in the composition of matter to colonize said plant root.
1. A composition of matter comprising:
about 30,000 propagules of Mycorrhizae in about five gallons of water;
about one gallon of an about 15 to about 40 percent aqueous solution of a phytate;
about 6.75×10 8 to about 4.20×10 9 colony forming units (cfu) of Trichoderma virens ; and
about 1.35×10 10 to about 8.40×10 10 cfu of Bacillus amyloliquefaciens.
2. A composition of matter comprising:
a Trichoderma virens component;
a Bacillus amyloliquefaciens component;
a mycorrhizal fungus or mycorrhizal fungi component; and
a phytate or phytic acid component.
3. A method for increasing plant yield comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Mycorrhizae, Trichoderma virens and Bacillus amyloliquefaciens to colonize said plant root.
4. The method of claim 3 wherein placing the composition of claim 1 in the vicinity of the plant root comprises
applying said Trichoderma virens in a concentration ranging from about 1.0×10 7 to about 1.0×10 11 viable Trichoderma virens strain G1-3 and/or Trichoderma virens strain G1-21 spores per gram of said Trichoderma virens and at an application rate of about 1.35 g per acre or 1.35 ml per acre.
5. The method of claim 3 wherein placing the composition of claim 1 in the vicinity of the plant root comprises
applying said Bacillus amyloliquefaciens in a concentration ranging from about 1×10 7 to about 5×10 11 cfu/g or cfu/ml of viable Bacillus amyloliquefaciens strain BAA-3 and/or Bacillus amyloliquefaciens strain FZB24 spores per gram of said Bacillus amyloliquefaciens and at an application rate of about 1.35 g or 1.35 ml per acre.
6. The method of claim 3 wherein said composition is placed in the vicinity of said plant root by application to a pre-planted seed, by in-furrow application as a seed is being planted, or by broadcasting over a seed row.
7. A method for improving soil aggregation and soil quality comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Mycorrhizae, Trichoderma virens and Bacillus amyloliquefaciens to colonize said plant root.
8. A method for increasing the yield of a crop, said method comprising:
applying a composition of matter to each acre of cropland;
wherein said composition of matter comprises:
about 30,000 propagules of Mycorrhizae in about five gallons of water;
about one gallon of an about 15 to about 40 percent aqueous solution of a phytate;
about 6.75×10 8 to about 4.20×10 9 colony forming units (cfu) of Trichoderma virens ; and
about 1.35×10 10 to about 8.40×10 10 cfu of Bacillus amyloliquefaciens.
9. A method for increasing plant yield comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Mycorrhizae to colonize said plant root.
10. A method for increasing plant yield comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Trichoderma virens and said Bacillus amyloliquefaciens to colonize said plant root.
11. A method for improving soil aggregation and soil quality comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Mycorrhizae to colonize said plant root.
12. A method for improving soil aggregation and soil quality comprising:
placing the composition of claim 1 in the vicinity of a plant root in a manner that allows said Trichoderma virens said Bacillus amyloliquefaciens to colonize said plant root.
13. A method for increasing plant yield comprising:
placing the composition of claim 2 in the vicinity of a plant root in a manner that allows said mycorrhizal fungus or mycorrhizal fungi, Trichoderma virens and Bacillus amyloliquefaciens to colonize said plant root.
14. A method for increasing plant yield comprising:
placing the composition of claim 2 in the vicinity of a plant root in a manner that allows said mycorrhizal fungus or mycorrhizal fungi to colonize said plant root.
15. A method for increasing plant yield comprising:
placing the composition of claim 2 in the vicinity of a plant root in a manner that allows said Trichoderma virens and said Bacillus amyloliquefaciens to colonize said plant root.
16. The method of claim 13 wherein said composition is placed in the vicinity of said plant root by application to a pre-planted seed, by in-furrow application as a seed is being planted, or by broadcasting over a seed row.
17. The composition of claim 1 wherein said cfu of Trichoderma virens comprise cfu of Trichoderma virens strain G1-3 and/or Trichoderma virens strain G1-21.
18. The composition of claim 2 wherein said Trichoderma virens component comprises Trichoderma virens strain G1-3 and/or Trichoderma virens strain G1-21.
19. The composition of claim 1 wherein said cfu of Bacillus amyloliquefaciens comprise cfu of Bacillus amyloliquefaciens strain BAA-3 and/or Bacillus amyloliquefaciens strain FZB24.
20. The composition of claim 2 wherein said Bacillus amyloliquefaciens component comprises Bacillus amyloliquefaciens strain BAA-3 and/or Bacillus amyloliquefaciens strain FZB24.