Basement Membrane Compositions and Applications Thereof
The invention relates to cell support compositions comprising a basement membrane extract isolated from cardiac or smooth muscle. The invention also relates to methods of using the cell support compositions for supporting cellular functions.
1 . A cell support composition comprising in parts by weight 5-50% laminin, wherein laminin type 1 and laminin type 3 are not present in any amount, 5-50% collagen, 1-10% nidogen, 5-60% heparan sulfate proteoglycan, and 1-10% entactin.
2 . The cell support composition of claim 1 , wherein laminin type 5 is not present in any amount.
3 . The cell support composition of claim 1 , wherein collagen type IV is present.
4 . The cell support composition of claim 1 , wherein collagen type I is present.
5 . The cell support composition of claim 1 , wherein collagen type VI is present.
6 . The cell support composition of claim 3 , wherein collagen type VI is present.
7 . The cell support composition of claim 4 , wherein collagen type VI is present.
8 . The cell support composition of claim 7 , wherein collagen type VI is present.
9 . The cell support composition of claim 1 , wherein the heparan sulfate proteoglycan comprises perlecan, wherein the perlecan is present in the cell support composition in an amount from about 5% to about 60% by weight.
10 . The cell support composition of claim 1 , further comprising titan protein.
11 . The cell support composition of claim 1 , further comprising myosin 7.
12 . The cell support composition of claim 1 , further comprising actinin protein.
13 . The cell support composition of claim 1 , wherein the cell support composition promotes the maintenance of plasticity of stem cells in cell culture.
14 . The cell support composition of claim 1 , wherein the cell support composition polymerizes at a temperature of at least about 37° C. within about 1 hour.
15 . The cell support composition of claim 1 , wherein the cell support composition is polymerized.
16 . The cell support composition of claim 15 , wherein the polymerized cell support composition is in form of nanofibers.
17 . A cell culture surface comprising the cell support composition of claim 15 .
18 . A method of maintaining the plasticity of stem cells, comprising culturing the stem cells on a cell culture surface comprising the cell support composition of claim 1 , wherein the cell support composition has been polymerized prior to culturing the stem cells thereon.
19 . The method of claim 18 , wherein the stem cells are adult stem cells (ASCs).
20 . The method of claim 18 , wherein the stem cells are embryonic stem cells (ESCs).
21 . The method of claim 18 , wherein the stem cells are induced pluripotent cells (iPCs).
22 . The method of claim 18 , wherein the cell support composition is in the form of nanofibers.
23 . A method of treating tissue comprising administering to a subject in need of treatment the cell support composition of claim 1 .