Method for manufacturing cell culture substrate
View Patent ↗A method for manufacturing a cell culture substrate obtained by forming a coating layer of a polymer on the surface of a substrate, wherein the polymer is formed by polymerizing a monomer component containing a nitrogen atom-containing monomer represented by formula (I): wherein R 1 is hydrogen atom or methyl group, R 2 is an alkylene group having 1 to 6 carbon atoms, each of R 3 and R 4 is independently an alkyl group having 1 to 4 carbon atoms, R 5 is an alkylene group having 1 to 4 carbon atoms, and Y is oxygen atom or —NH— group, includes irradiating the polymer with ion beam at a predetermined position of the coating layer of the polymer, to remove the coating layer of the polymer irradiated with the ion beam.
1. A method for manufacturing a cell culture substrate comprising a substrate and a coating layer of a polymer formed on the surface of the substrate, said method comprising the steps of,
polymerizing a monomer component comprising a nitrogen atom-containing monomer represented by the formula (I) on the surface of a substrate having hydroxyl groups thereon, to form a coating layer of the polymer on said surface:
wherein R 1 is hydrogen atom or methyl group, R 2 is an alkylene group having 1 to 6 carbon atoms, each of R 3 and R 4 is independently an alkyl group having 1 to 4 carbon atoms, R 5 is an alkylene group having 1 to 4 carbon atoms, and Y is oxygen atom or —NH— group, and
irradiating the coating layer of the polymer at a predetermined position with ion beam, to remove the coating layer of the polymer irradiated with the ion beam at the predetermined position.
2. The method for manufacturing a cell culture substrate according to claim 1 , wherein the monomer component further comprises at least one copolymerizable monomer selected from the group consisting of an alkyl (meth)acrylamide-based monomer, a polyethylene glycol-based monomer, a polypropylene glycol-based monomer, a sugar backbone-containing monomer, a phosphobetaine-based monomer and a sulfobetaine-based monomer.
3. A cell culture substrate obtained by the method for manufacturing a cell culture substrate according to claim 1 .
4. The method for manufacturing a cell culture substrate according to claim 1 , wherein the substrate having hydroxyl groups is a glass substrate.
5. The method for manufacturing a cell culture substrate according to claim 4 , wherein the glass substrate is a glass plate.
6. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 2 is a methylene group.
7. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 2 is an ethylene group.
8. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 3 and R 4 are methyl groups.
9. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 3 and R 4 are ethyl groups.
10. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 5 is a methylene group.
11. The method for manufacturing a cell culture substrate according to claim 1 , wherein R 5 is an ethylene group.