IP Library › Granted Patent US 9,267,117
Granted Patent B2
US 9,267,117 · App. 13/826,395 · Granted Feb 23, 2016

Mapping cytosine modifications

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Quick Facts
Patent No.
US 9,267,117
App. No.
13/826,395
Granted
Feb 23, 2016
Kind
B2
Abstract

Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.

Claims (6)

1. A composition comprising a reducing agent capable of converting 5-formylcytosine or 5-carboxycytosine to 5-hydroxymethylcytosine; T4 β-glucosyltransferase (BGT) and a UDP derivative selected from the group consisting of UDP-glucose and UDP-azidoglucose.

2. A composition according to claim 1 , further comprising a nucleic acid.

3. A kit comprising a reducing agent capable of converting 5-formylcytosine or 5-carboxycytosine to 5-hydroxymethylcytosine; T4 β-glucosyltransferase (BGT); and a UDP derivative selected from the group consisting of UDP-glucose and UDP-azidoglucose.

4. A kit according to claim 3 , further comprising UDP-glucosamine.

5. A kit according to claim 3 , further comprising a nucleic acid.

6. A kit according to any claim 3 , further comprising an oxidizing agent that is capable of oxidizing 5-methylcytosine to 5-hydroxymethylcytosine.

Assignments (3)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
CONFIRMATORY LICENSE Recorded Apr 27, 2015
From: NEW ENGLAND BIOLABS, INC.
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 035508/0547 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jun 19, 2013
From: GUAN, SHENGXI; DAI, NAN; ZHU, ZHENYU; CORREA, IVAN R., JR.; QUIMBY, AINE; BORGARO, JANINE
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 030644/0346 →