METHOD FOR IMMOBILIZING A PROTEIN ON SELF-ASSEMBLED MONOLAYER
One molecule of the amino acid selected from the five kinds of amino acids consisting of cysteine, lysine, histidine, phenylalanine, and glycine is interposed between a self-assembled monolayer and a molecule of a protein. A method for immobilizing an protein on a self-assembled monolayer includes the following steps (a) and (b) in this order: a step (a) of preparing a substrate including one molecule of an amino acid and the self-assembled monolayer and a step (b) of supplying the protein to the substrate to form a peptide bond represented by a predetermined chemical formula as a result of reaction between the carboxyl group of the one molecular of the amino acid and the amino group of the protein.
1 . A method for immobilizing a protein on a self-assembled monolayer, the method comprising:
a step (a) of preparing a substrate comprising one molecule of an amino acid and the self-assembled monolayer, wherein
the one molecule of the amino acid is bound to the self-assembled monolayer through a peptide bond represented by the following chemical formula (I):
where R represents a side chain of the one molecule of the amino acid,
the one molecule of the amino acid is selected from the group of amino acids consisting of cysteine, lysine, histidine, phenylalanine, and glycine; and
a step (b) of supplying the protein to the substrate to form a peptide bond represented by the following chemical formula (II) as a result of reaction between a carboxyl group of the one molecule of the amino acid and an amino group of the protein:
where R represents the side chain of the one molecule of the amino acid.
2 . The method according to claim 1 , wherein the step (a) comprises:
a step (a1) of preparing the substrate comprising the self-assembled monolayer on a surface thereof, the self-assembled monolayer having a carboxyl group at one end; and
a step (a2) of supplying the one molecule of the amino acid to form the peptide bond represented by the chemical formula (I) as a result of reaction between the carboxyl group of the one end of the self-assembled monolayer and an amino group of the one molecule of the amino acid.
3 . The method according to claim 1 , further comprising, between the step (a) and the step (b):
a step (ab) of activating a carboxyl group of the one molecule of the amino acid with a mixture of N-Hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride.
4 . The method according to claim 2 , further comprising, between the step (a1) and the step (a2):
a step (a1a) of activating the carboxyl group of the self-assembled monolayer with a mixture of N-Hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride.
5 . The method according to claim 1 , wherein the chemical formula (II) is represented by the following chemical formula (III):
where R represents the side chain of the one molecule of the amino acid.
6 . A sensor comprising:
a self-assembled monolayer;
one molecule of an amino acid; and
a protein, wherein:
the one molecule of the amino acid is interposed between the self-assembled monolayer and the protein,
the protein is bound to the self-assembled monolayer through two peptide bonds represented by the following chemical formula (II):
where R represents a side chain of the one molecule of the amino acid, and
the one molecule of the amino acid is selected from the group of amino acids consisting of cysteine, lysine, histidine, phenylalanine, and glycine.
7 . The sensor according to claim 6 , wherein the chemical formula (II) is represented by the following chemical formula (III):
where R represents the side chain of the one molecule of the amino acid.
8 . A method for detecting or quantifying a target substance contained in a sample with use of a sensor, the method comprising:
a step (a) of preparing the sensor comprising a self-assembled monolayer, one molecule of an amino acid, and a protein, wherein
the one molecule of the amino acid is interposed between the self-assembled monolayer and the protein,
the protein is bound to the self-assembled monolayer through two peptide bonds represented by the following chemical formula (II):
where R represents a side chain of the one molecule of the amino acid, and
the one molecule of the amino acid is selected from the group of amino acids consisting of cysteine, lysine, histidine, phenylalanine, and glycine;
a step (b) of supplying the sample to the sensor to bind the target substance to the protein; and
a step (c) of detecting the target substance bound in the step (b), or quantifying the target substance contained in the sample from an amount of the target substance bound in the step (b).
9 . The method according to claim 8 , wherein the chemical formula (II) is represented by the following chemical formula (III):
where R represents the side chain of the one molecule of the amino acid.
10 . The method according to claim 1 , wherein the protein does not include Protein A, streptavidin, glucose oxidase, antibody or albumin.
11 . The sensor according to claim 6 , wherein the protein does not include Protein A, streptavidin, glucose oxidase, antibody or albumin.
12 . The method according to claim 8 , wherein the protein does not include Protein A, streptavidin, glucose oxidase, antibody or albumin.