Methods and materials for treatment of pompe's disease
This document relates to molecular complexes having acid alpha glucosidase activity and at least one modification that results in enhanced ability of the molecular complex to be transported to the interior of a mammalian cell.
1. A method for making a molecular complex having acid alpha glucosidase (GAA) activity, said method comprising contacting a GAA protein having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:1 with a protease having at least 85% sequence identity to the amino acid sequence set forth in SEQ ID NO:8, wherein said protease cleaves said protein at one or more sites between amino acid 60 and amino acid 65 of SEQ ID NO:1 to produce a molecular complex having GAA activity and comprising at least two polypeptides.
2. The method of claim 1 , wherein said contacting is performed in vitro.
3. The method of claim 1 , further comprising proteolysis of the protein at one or more sites between amino acid 719 and amino acid 746 of the amino acid sequence set forth in SEQ ID NO:1.
4. The method of claim 1 , further comprising proteolysis of the protein at one or more sites between amino acid 137 and amino acid 151 of the amino acid sequence set forth in SEQ ID NO:1.
5. The method of claim 1 , wherein the contacting occurs in a recombinant fungal cell.
6. The method of claim 5 , wherein the fungal cell is a cell of Yarrowia lipolytica, Arxula adeninivorans , or a methylotrophic yeast.
7. The method of claim 6 , wherein the methylotrophic yeast is a cell of a genus selected from the group consisting of Candida, Hansenula, Oogataea, Pichia , and Torulopsis.
8. The method of claim 1 , further comprising altering the molecular complex with at least one modification that results in enhanced ability of the molecular complex to be transported to the interior of a mammalian cell, wherein:
the at least one modification comprises the recognition domain of human insulin-like growth factor II; or
at least one of said polypeptides comprises one or more phosphorylated N-glycans and wherein said modification comprises uncapping and demannosylation of at least one phosphorylated N-glycan.
9. The method of claim 8 , wherein at least 40% of the N-glycans on said polypeptide are uncapped and demannosylated.
10. The method of claim 8 , wherein at least 60% of the N-glycans on said polypeptide are uncapped and demannosylated.
11. The method of claim 8 , wherein at least 80% of the N-glycans on said polypeptide are uncapped and demannosylated.
12. The method of claim 8 , wherein at least 90% of the N-glycans on said polypeptide are uncapped and demannosylated.