Solid matrix for the storage of biological samples
View Patent ↗The present invention relates to a method for storage and subsequent lysis of a sample in which the sample is immobilized on a solid support. The solid matrix is embedded with a low concentration of both a chaotropic salt and a surfactant which act synergistically to efficiently store and lyse a biological sample.
1. A solid matrix for storing and/or lysing a biological sample comprising a surfactant and a chaotropic salt, wherein the concentration of the chaotropic salt to surfactant is 0.1M:0.1M, the surfactant is Triton X100, and the chaotropic salt is a guanidinium salt.
2. The solid matrix of claim 1 , wherein the biological sample is selected from the group consisting of a cellular sample, blood, serum, semen, cerebral spinal fluid, synovial fluid, lymphatic fluid, saliva, buccal, cervical cell, vaginal cell, urine, faeces, hair, skin and muscle.
3. The solid matrix of claim 1 , wherein the solid matrix is selected from the group consisting of alginate, glass, glass fiber, glass microfiber, silica, silica gel, silica oxide, cellulose, nitrocellulose, carboxymethylcellulose, polyester, polyamide, carbohydrate polymers, polypropylene, polytetraflurorethylene, polyvinylidinefluoride, wool and porous ceramics.
4. The solid matrix of claim 1 , wherein the solid matrix is a cellulose based matrix.
5. The solid matrix of claim 4 , additionally wherein said cellulose based matrix is in the form of a pre punched disc.
6. The solid matrix of claim 4 , additionally wherein the cellulose based matrix is in the form of a pre punched disc.
7. A method of storing and/or lysing a biological sample comprising the steps:
i) contacting a solid matrix of any preceding claim with a biological sample; and
ii) storing said solid matrix with said biological sample,
wherein the concentration of the chaotropic salt to surfactant is 0.1M:0.1M, the surfactant is Triton X100, and the chaotropic salt is a guanidinium salt.
8. The method of claim 7 , further comprising a step iii) of recovering the cell lysate from the solid matrix.
9. The method of claim 7 , wherein the biological sample is selected from a group consisting of a cellular sample, blood, serum, semen, cerebral spinal fluid, synovial fluid, lymphatic fluid, saliva, buccal, cervical cell, vaginal cell, urine, faeces, hair, skin and muscle.
10. A method of manufacturing a solid matrix comprising the steps:
i) dipping a solid matrix into a mixture of a surfactant and a chaotropic salt wherein the concentration of said chaotropic salt to surfactant is 0.1M:0.1M, the surfactant is Triton X100, and the chaotropic salt is a guanidinium salt; and
ii) allowing the solid matrix to dry.
11. The method of claim 10 , wherein the solid matrix is selected from the group consisting of alginate, glass, glass fiber, glass microfiber, silica, silica gel, silica oxide, cellulose, nitrocellulose, carboxymethylcellulose, polyester, polyamide, carbohydrate polymers, polypropylene, polytetraflurorethylene, polyvinylidinefluoride, wool or porous ceramics.
12. A kit for storing and amplifying nucleic acid comprising the solid matrix of claim 1 and instructions for use thereof.