Solid phase peptide synthesis processes and associated systems
Systems and processes for performing solid phase peptide synthesis are generally described. Solid phase peptide synthesis is a known process in which amino acid residues are added to peptides that have been immobilized on a solid support. In certain embodiments, the inventive systems and methods can be used to perform solid phase peptide synthesis quickly while maintaining high yields. Certain embodiments relate to processes and systems that may be used to heat, transport, and/or mix reagents in ways that reduce the amount of time required to perform solid phase peptide synthesis.
1. A process for adding amino acid residues to peptides, comprising:
heating a stream comprising amino acids such that the temperature of the amino acids is increased by at least about 1° C.; and
exposing the heated amino acids to a plurality of peptides immobilized on a solid support,
wherein the heating step is performed prior to and within about 30 seconds of exposing the heated amino acids to the peptides.
2. The process of claim 1 , wherein the solid support comprises polystyrene and/or polyethylene glycol.
3. The process of claim 1 , wherein the solid support comprises a resin.
4. The process of claim 3 , wherein the solid support comprises a microporous polystyrene resin.
5. The process of claim 1 , wherein the solid support is contained within a packed column and/or a fluidized bed.
6. The process of claim 1 , wherein an amino acid residue is added to at least about 99% of the immobilized peptides after exposing the heated amino acids to the immobilized peptides.
7. The process of claim 1 , wherein multiple copies of the amino acid residue are bonded to fewer than about 1% of the immobilized peptides after exposing the heated amino acids to the immobilized peptides.
8. The process of claim 6 , wherein adding an amino acid residue to the immobilized peptides comprises adding a single amino acid residue to the immobilized peptides.
9. The process of claim 6 , wherein adding an amino acid residue to the immobilized peptides comprises adding a peptide comprising two or more amino acid residues to the immobilized peptides.
10. The process of claim 1 , wherein the amino acids comprise activated amino acids.
11. The process of claim 1 , wherein the amino acids comprise protection groups.
12. The process of claim 11 , wherein amine groups of the amino acids are protected.
13. The process of claim 11 , wherein the protection groups comprise fluorenylmethyloxycarbonyl protection groups.
14. The process of claim 11 , wherein the protection groups comprise tert-butyloxycarbonyl protection groups.
15. The process of claim 11 , wherein the protection groups comprise allyloxycarbonyl protection groups.
16. The process of claim 11 , wherein the protection groups comprise carboxybenzyl protection groups.