Ruggedized apparatus for analysis of nucleic acid and proteins
The invention provides methods and systems for ruggedizing a nucleic acid analyzing apparatus. The ruggedized apparatus can be used reliably and effectively in uncontrolled environments, such as, for example at a crime scene to collect and analyze forensic data, as well as in semi-controlled environments, such as, for example at a point of care location.
1. An electrophoresis system for finding the position of at least one separation channel, the system comprising:
(A) a removable test module comprising a transparent material that allows at least a part of a fluorescence excitation energy beam to interact with said at least one capillary disposed within said removable test module, said at least one capillary, further comprising a separation channel and a detection zone; and
(B) an electrophoresis instrument capable of receiving said removable test module said electrophoresis instrument comprising;
(1) a fluorescence excitation and detection system further comprising: a fluorescence excitation beam source, producing, in use, said fluorescence excitation energy beam, that is capable of interacting with said at least one capillary by deflection and travelling along the length of said removable test module, and of intersecting said detection zone of said at least one capillary when inserted into said electrophoresis instrument, and
(2) at least one light detector positioned on a first side of said removable test module when inserted into said electrophoresis instrument, and further positioned to detect a signal from light that has travelled through said at least one capillary, and which has been deflected thereby, as said signal is exiting said removable test module, said fluorescence excitation beam source configured such that said fluorescence excitation energy beam is capable of intersecting the detection zone of said at least one capillary when inserted into said electrophoresis instrument; and
(3) a processor configured to direct said fluorescence excitation energy beam source to transmit said fluorescent excitation energy beam towards said detection zone and to receive data collected by said at least one light detector,
wherein in use, at least a part of said fluorescence excitation energy beam is transmitted through the removable test module, and deflected light travels within the test module and along its length and is detected by said at least one light detector to generate an intensity pattern that is used by the processor to determine the position of the middle of each of said at least one channel.
2. The system as claimed in claim 1 , wherein the fluorescence excitation beam is a laser beam.
3. The system as claimed in claim 1 , wherein said at least one light detector is a photodiode.
4. The system as claimed in claim 1 comprising a first detector and a second detector placed on either side of said removable test module.
5. The system as claimed in claim 4 wherein said first and second detectors are photodiodes.
6. The system of claim 1 or 2 or 3 or 5 where said at least one capillary is microfluidic.
7. The system of claim 4 wherein said at least one capillary is microfluidic.