Synergistic combinations of organic acid useful for controlling microoganisms in industrial processes
View Patent ↗The present invention provides a method of controlling bacterial contamination using synergistic interactions of antimicrobials. The invention utilizes a combination of organic acids where the combined antimicrobial effect is synergistic.
1. A method of controlling undesirable microorganism concentration in an aqueous system employed in a fermentation process, the method comprising the steps of:
(a) introducing a fermentable carbohydrate to an aqueous solution;
(b) introducing at least one yeast to said aqueous solution;
(c) introducing a first organic acid and a second organic acid into the aqueous system, wherein the aqueous system comprises undesirable microorganisms wherein at least one undesirable microorganism is selected from the group consisting of lactic acid producing bacteria and acetic acid producing bacteria; and
wherein the first organic acid is selected from citric acid, benzoic acid, or their salts; and wherein
the first organic acid and a second organic acid is added to at least one stage in the fermentation process selected from liquefaction, propagation, conditioning and fermentation.
2. The method of claim 1 wherein the first organic acid is citric acid.
3. The method of claim 1 wherein the first organic acid comprises citric acid or its salt, and wherein the at least one second organic acid is selected from the group consisting of propionic acid, benzoic acid, or their salts.
4. The method of claim 1 wherein the first organic acid has a dosage rate of at least 100 ppm in the aqueous system being treated.
5. The method of claim 1 wherein the first organic acid has a dosage rate of at least 100 ppm and up to about 12500 ppm in the aqueous system being treated.
6. The method of claim 1 wherein the at least one first organic acid comprises citric acid, the at least one second organic acid comprises propionic acid or its salt, in which the ratio of citric acid to propionic acid is from 64:1 to 1:16 and the amount of the first organic acid is the aqueous system to be treated is from 100 to 12500 ppm.
7. The method of claim 1 wherein the at least one first organic acid comprises citric acid, the at least one second organic acid comprises benzoic acid or its salt, wherein the ratio of citric acid to benzoic acid is from 8:1 to 1:32, and the amount of the first organic acid is the aqueous system to be treated is from 100 to 12500 ppm.
8. A method of controlling undesirable microorganism concentration in an aqueous fluid solution employed in a fermentation process, the method comprising the steps of:
(a) introducing a fermentable carbohydrate to an aqueous solution;
(b) introducing at least one desirable microorganism which is capable of fermenting carbohydrate to said aqueous solution;
(c) introducing at least one first organic acid into said aqueous solution; and
(d) introducing at least one second organic acid into said aqueous solution, wherein the aqueous system comprises undesirable microorganisms wherein at least one undesirable microorganism is selected from the group consisting of lactic acid producing bacteria and acetic acid producing bacteria; and
wherein the first organic acid is selected from citric acid, benzoic acid, or their salts; and wherein
the first organic acid and the second organic acid is added to at least one stage in the fermentation process selected from liquefaction, propagation, conditioning and fermentation.
9. The method of claim 8 wherein the first organic acid is selected from the group consisting of citric acid, benzoic acid, and their salts.
10. A method of controlling undesirable microbial growth in fermentation broths or industrial fermentation processes or systems, the method consisting of the step of adding to a fermentation broth or industrial fermentation processes or system (a) at least one first organic acid and
(b) at least one second organic acid wherein the first organic acid is different from the second organic acid, wherein the aqueous system comprises undesirable microorganisms wherein at least one undesirable microorganism is selected from the group consisting of lactic acid producing bacteria and acetic acid producing bacteria; and
wherein the first organic acid is selected from the group consisting of citric acid, benzoic acid and their salts; and wherein
the first organic acid and a second organic acid is added to at least one stage in the fermentation process selected from liquefaction, propagation, conditioning and fermentation.
11. The method of claim 10 wherein the first organic acid is citric acid.
12. The method of claim 10 wherein the first organic acid comprises citric acid or its salt, and wherein the at least one second organic acid is selected from the group consisting of propionic acid, benzoic acid, or their salts.
13. The method of claim 10 wherein the first organic acid has a dosage rate of at least 100 ppm in the aqueous system being treated.
14. The method of claim 10 wherein the first organic acid has a dosage rate of at least 100 ppm and up to about 12500 ppm in the aqueous system being treated.
15. The method of claim 10 wherein the at least one first organic acid comprises citric acid, the at least one second organic acid comprises propionic acid or its salt, in which the ratio of citric acid to propionic acid is from 64:1 to 1:16 and the amount of the first organic acid is the aqueous system to be treated is from 100 to 12500 ppm.
16. A method of controlling undesirable microorganism concentration in an aqueous system employed in a fermentation process, the method comprising the steps of:
(a) introducing a fermentable carbohydrate to an aqueous solution;
(b) introducing at least one yeast to said solution;
(c) introducing a first organic acid and a second organic acid said into the aqueous system, wherein the aqueous system comprises undesirable microorganisms wherein at least one undesirable microorganism is selected from the group consisting of lactic acid producing bacteria and acetic acid producing bacteria; and
wherein the first organic acid is selected from citric acid, benzoic acid, or their salts; and wherein
the first organic acid is added to a location in the fermentation process selected from the slurry tank, cook vessels, the mash, the propagation tanks, the conditioning tanks, the starter tanks, heat exchangers, the fermentation tanks during the simultaneous saccharification and fermentation stage and the piping between any of these units.
17. The method of claim 16 wherein the first organic acid is citric acid.
18. The method of claim 16 wherein the first organic acid comprises citric acid or its salt, and wherein the at least one second organic acid is selected from the group consisting of propionic acid, benzoic acid, or their salts.
19. The method of claim 16 wherein the first organic acid has a dosage rate of at least 100 ppm and up to about 12500 ppm in the aqueous system being treated.
20. The method of claim 16 wherein the at least one first organic acid comprises citric acid, the at least one second organic acid comprises benzoic acid or its salt, wherein the ratio of citric acid to benzoic acid is from 8:1 to 1:32, and the amount of the first organic acid is the aqueous system to be treated is from 100 to 12500 ppm.