Enhanced Fermentation From Pretreatment Products
Provided are methods and compositions for higher yields through the addition of pretreatment/hydrolysis extracts in the fermentation process. These fractions increase the rate of growth for biocatalysts and result in improved composition of fermentation end products.
1 .- 320 . (canceled)
321 . A method of producing one or more fermentation end-products comprising:
(a) contacting a saccharide solution comprising C5 and/or C6 monosaccharides, and particulate solids, with a cell culture; and
(b) allowing sufficient time for cells in the cell culture to produce one or more fermentation end-products comprising one or more polyols, fatty acids, triacylglycerols, or a combination thereof; wherein a greater yield of the one or more polyols, fatty acids, or triacylglycerols is produced in comparison to fermentation of a saccharide solution comprising a lower level of the particulate solids or one or more osmotic agents.
322 . The method of claim 321 , wherein the particulate solids comprise lignin, cellulose, hemicellulose, or a combination thereof.
323 . The method of claim 321 , wherein the particulate solids are residual solids from pretreating and hydrolyzing a biomass.
324 . The method of claim 321 , wherein the saccharides solution further comprises one or more osmotic agents.
325 . The method of claim 324 , wherein the one or more osmotic agents comprise one or more salts, acid solubilized lignin, one or more fatty acids, one or more metal ions, one or more trace elements, one or more acids, one or more bases, ash, one or more organic acids, one or more alcohols, or a combination thereof.
326 . The method of claim 321 , further comprising adding exogenous particulate solids or one or more exogenous osmotic agents to the saccharide solution.
327 . The method of claim 321 , wherein the cell culture comprises plant cells, bacterial cells, yeast cells, algal cells, or a combination thereof.
328 . The method of claim 321 , wherein the one or more fermentation end-products comprises glycerol.
329 . The method of claim 321 , wherein the saccharide solution was produced by pretreating and hydrolyzing a biomass composition comprising cellulosic, hemicellulosic, and/or lignocellulosic material.
330 . A method of producing one or more fermentation end-products comprising:
(a) contacting a saccharide solution comprising C5 and/or C6 monosaccharides, and one or more osmotic agents, with a cell culture; and
(b) allowing sufficient time for cells in the cell culture to produce one or more fermentation end-products comprising one or more polyols, fatty acids, triacylglycerols, or a combination thereof; wherein a greater yield of the one or more polyols, fatty acids, or triacylglycerols is produced in comparison to fermentation of a saccharide solution comprising a lower level of the one or more osmotic agents.
331 . The method of claim 330 , wherein the one or more osmotic agents comprise one or more salts, acid solubilized lignin, one or more fatty acids, one or more metal ions, one or more trace elements, one or more acids, one or more bases, ash, one or more organic acids, one or more alcohols, or a combination thereof.
332 . A system for producing an increased yield of polyols, fatty acids, and/or triacylglycerols, the system comprising:
(a) a fermentation vessel;
(b) a saccharide solution comprising C5 and/or C6 monosaccharides, and particulate solids or one or more osmotic agents; and
(c) a cell culture comprising cells that produce one or more polyols, fatty acids and/or triacylglycerols from the C5 and/or C6 monosaccharides in a greater yield than from an equivalent amount of the C5 and/or C6 monosaccharides with a lower level of the particulate solids or the one or more osmotic agents.
333 . The system of claim 332 , wherein the saccharide solution comprises the particulate solids.
334 . The system of claim 333 , wherein the particulate solids comprise lignin, cellulose, hemicellulose, or a combination thereof.
335 . The system of claim 333 , wherein the particulate solids are residual solids from pretreating and hydrolyzing a biomass.
336 . The system of claim 332 , wherein the saccharide solution comprises the one or more osmotic agents.
337 . The system of claim 336 , wherein the one or more osmotic agents comprise one or more salts, acid solubilized lignin, one or more fatty acids, one or more metal ions, one or more trace elements, one or more acids, one or more bases, ash, one or more organic acids, one or more alcohols, or a combination thereof.
338 . The system of claim 332 , wherein the cell culture comprises plant cells, bacterial cells, yeast cells, algal cells, or a combination thereof.
339 . The system of claim 332 , wherein the one or more fermentation end-products comprises glycerol.
340 . The system of claim 332 , wherein the saccharide solution was produced by pretreating and hydrolyzing a biomass composition comprising cellulosic, hemicellulosic, and/or lignocellulosic material.