System and cartridge for efficient nucleic acid testing
A disposable and inexpensive biological diagnostic cartridge for the amplification and detection of nucleic acids includes a configuration having a reaction pouch for amplification which is compressed by a flexible pump pouch for detection of the amplified reaction.
1. A biological diagnostic cartridge system, comprising:
a cartridge comprising:
an inlet;
a flexible reaction pouch downstream of the inlet, the flexible reaction pouch for housing a reaction liquid sample, the flexible reaction pouch having a first side and a second side, the first side directly contacting a rigid substrate;
a flexible pump pouch for housing a thermally conductive liquid and compressing the flexible reaction pouch, the flexible pump pouch directly contacting and covering all of the second side of the flexible reaction pouch; and
a heating element in direct contact with the flexible pump pouch, the flexible pump pouch positioned between the heating element and the second side of the flexible reaction pouch.
2. The cartridge system of claim 1 , wherein the cartridge further comprises:
a detection chamber downstream of the flexible reaction pouch;
a first fluid path from the inlet to the flexible reaction pouch; and
a second fluid path from the flexible reaction pouch to the detection chamber.
3. The cartridge system of claim 2 , wherein each of the first and second fluid paths is one-directional.
4. The cartridge system of claim 2 , wherein the cartridge further comprises a first flow strip positioned in the detection chamber.
5. The cartridge system of claim 4 , wherein the cartridge further comprises a second inlet, a second flexible reaction pouch downstream of the second inlet, a second flexible pump pouch adapted to compress the second flexible reaction pouch, a second detection chamber downstream of the second flexible reaction pouch.
6. The cartridge system of claim 5 , wherein the cartridge further comprises a second flow strip arranged antiparallel or parallel to a first flow strip.
7. The cartridge system of claim 1 , wherein the cartridge further comprises an outlet, the outlet comprising an outlet one-way passive check valve having a cracking pressure, wherein when the flexible reaction pouch houses the reaction liquid, the flexible reaction pouch has a fluid pressure, and wherein the cracking pressure of the outlet one-way passive check valve is greater than the fluid pressure of the flexible reaction pouch.
8. The cartridge system of claim 7 , wherein the cracking pressure of the outlet one-way passive check valve is from about 2 to about 8 psi.
9. The cartridge system of claim 7 , wherein the inlet comprises an inlet one-way passive check valve having a cracking pressure lower than the cracking pressure of the outlet one-way passive check valve.
10. The cartridge system of claim 7 , wherein the cartridge further comprises a detection chamber, wherein the outlet one-way passive check valve is downstream of the reaction pouch and upstream of the detection chamber.
11. The cartridge system of claim 1 , further comprising
a housing; and
a pump adapted to inflate the flexible pump pouch, wherein the housing comprises a port for receiving the pump.
12. The cartridge system of claim 11 , wherein the pump comprises an electrolytic pump adapted to generate gas.
13. The cartridge system of claim 1 , wherein the cartridge further comprises a vent downstream of the inlet and upstream of the flexible reaction pouch.
14. The cartridge system of claim 13 , wherein the vent comprises a hydrophobic barrier.
15. A portable biological diagnostic system, comprising:
a cartridge, comprising:
an inlet adapted to receive a sample;
a flexible reaction pouch downstream of the inlet for housing a reaction liquid, the flexible reaction pouch having a first side and a second side, the first side directly contacting a rigid substrate;
a flexible pump pouch for housing a thermally conductive liquid and compressing the flexible reaction pouch, the flexible pump pouch directly contacting and covering all of the second side of the flexible reaction pouch; and
a detection chamber downstream of the flexible reaction pouch;
a pump to inflate the flexible pump pouch;
a heating element in direct contact with the flexible pump pouch, the flexible pump pouch positioned between the heating element and the second side of the flexible reaction pouch; and
a power source to drive the pump.
16. The system of claim 15 , wherein the pump comprises an electrolytic pump.
17. The system of claim 15 , further comprising a controller configured to control activation of the heating element and the power source.