Method of correcting for oxygen effect on test sensors

1. A method for correcting the oxygen effect in determining the concentration of glucose in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, and a counter electrode, the first working electrode including glucose oxidase, an oxidized form of a mediator and peroxidase, the second working electrode including glucose oxidase and the oxidized form of the mediator, the first working electrode, the second working electrode and the counter electrode being adjacent to the base;

contacting the test sensor to a meter to form an electrical connection;

placing the fluid on the test sensor;

measuring a first current from the first working electrode from a reduced form of the mediator;

measuring a second current from the second working electrode from the reduced form of the mediator; and

determining the concentration of glucose and correcting for the oxygen effect, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

2. The method of claim 1 , wherein the determination of the glucose concentration includes adding the second current measurement to the oxygen effect.

3. The method of claim 1 , wherein the fluid is blood.

4. The method of claim 1 , wherein the mediator is a ferricyanide derivative.

5. The method of claim 1 , wherein the mediator is a ruthenium (III) derivative or a ferricium derivative.

6. The method of claim 1 , wherein the mediator is an inorganic mediator.

7. The method of claim 1 , wherein the test sensor further includes a lid, the base and the lid assisting in forming one generally flat channel with a test sensor opening, wherein placing the fluid on the test sensor includes placing the fluid into the channel via the test sensor opening and further including contacting the fluid with the first working electrode and the second working electrode in the generally flat channels.

8. The method of claim 1 , wherein the test sensor further includes a lid and a spacer, the spacer being located between the base and the lid, the base and the lid assisting in forming one generally flat channel with a test sensor opening, wherein placing the fluid on the test sensor includes placing the fluid into the channel via the test sensor opening and further including contacting the fluid with the first working electrode and the second working electrode in the generally flat channel.

9. A method for correcting the oxygen effect in determining the concentration of glucose in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, and a counter electrode, the first working electrode including glucose oxidase, an oxidized form of mediator and peroxidase, the second working electrode including glucose oxidase and the oxidized form of the mediator in the absence of the peroxidase, the first working electrode, the second working electrode and the counter electrode being adjacent to the base;

contacting the test sensor to a meter to form an electrical connection;

placing the fluid on the test sensor;

measuring a first current from the first working electrode from a reduced form of the mediator;

measuring a second current from the second working electrode from the reduced form of the mediator; and

determining the concentration of glucose and correcting for the oxygen effect, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

10. The method of claim 9 , wherein the determination of the glucose concentration includes adding the second current measurement to the oxygen effect.

11. The method of claim 9 , wherein the fluid is blood.

12. The method of claim 9 , wherein the mediator is a ferricyanide derivative.

13. A method for correcting the oxygen effect in determining the concentration of cholesterol in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, and a counter electrode, the first working electrode including cholesterol oxidase, an oxidized form of mediator and peroxidase, the second working electrode including cholesterol oxidase and the oxidized form of the mediator, the first working electrode, the second working electrode and the counter electrode being adjacent to the base;

contacting the test sensor to a meter to form an electrical connection;

placing the fluid on the test sensor;

measuring a first current from the first working electrode from a reduced form of the mediator;

measuring a second current from the second working electrode from the reduced form of the mediator, and

determining the concentration of cholesterol and correcting for the oxygen effect, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

14. The method of claim 13 , wherein the determination of the cholesterol concentration includes adding the second current measurement to the oxygen effect.

15. The method of claim 13 , wherein the second working electrode including cholesterol oxidase and the mediator in the absence of the peroxidase.

16. A method for correcting the oxygen effect in determining the concentration of at least one of lactate, pyruvate or xanthine in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, and a counter electrode, the first working electrode including lactate, pyruvate or xanthine oxidase, an oxidized form of mediator and peroxidase, the second working electrode including lactate, pyruvate or xanthine oxidase and the oxidized form of the mediator, the first working electrode, the second working electrode and the counter electrode being adjacent to the base;

contacting the test sensor to a meter to form an electrical connection;

placing the fluid on the test sensor;

measuring a first current from the first working electrode from a reduced form of the mediator;

measuring a second current from the second working electrode from the reduced form of the mediator; and

determining the concentration of lactate, pyruvate or xanthine and correcting of the oxygen effect, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

17. The method of claim 16 , wherein the determination of the lactate, pyruvate or xanthine concentration includes adding the second current measurement to the oxygen effect.

18. A method for correcting the oxygen effect in determining the concentration of glucose in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, a counter electrode and a lid, the first working electrode including glucose oxidase, a mediator and peroxidase, the second working electrode including glucose oxidase and the mediator, the first working electrode, the second working electrode and the counter electrode being adjacent to the base, the base and the lid assisting in forming one generally flat channel with a test sensor opening;

contacting the test sensor to a meter to form an electrical connection;

placing the fluid into the channel via the test sensor opening;

contacting the fluid with the first working electrode and the second working electrode in the generally flat channel;

measuring a first current from the first working electrode;

measuring a second current from the second working electrode; and

determining the concentration of glucose and correcting for the oxygen effect by using the first current measurement and the second current measurement, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

19. The method of claim 18 , wherein the mediator is a ferricyanide derivative.

20. The method of claim 18 , wherein the test sensor further includes a spacer, the spacer being located between the base and the lid.

21. The method of claim 18 , wherein the determination of the glucose concentration includes adding the second current measurement to the oxygen effect.

22. A method for correcting the oxygen effect in determining the concentration of glucose in a fluid using an electrochemical test sensor, the method comprising the acts of:

providing a test sensor, the test sensor comprising a base, first and second working electrodes, a counter electrode and a lid, the first working electrode including glucose oxidase, a mediator and peroxidase, the second working electrode including glucose oxidase and the mediator in the absence of the peroxidase, the first working electrode, the second working electrode and the counter electrode being adjacent to the base, the base and the lid assisting in forming one generally flat channel with a test sensor opening;

contacting the test sensor to a meter to form an electrical connection,

placing the fluid into the channel via the test sensor opening;

contacting the fluid with the first working electrode and the second working electrode in the generally flat channel;

measuring a first current from the first working electrode;

measuring a second current from the second working electrode; and

determining the concentration of glucose and correcting for the oxygen effect by using the first current measurement and the second current measurement, the oxygen effect being determined by subtracting the first current measurement from the second current measurement.

23. The method of claim 22 , wherein the test sensor further includes a spacer, the spacer being located between the base and the lid.

24. The method of claim 22 , wherein the determination of the glucose concentration includes adding the second current measurement to the oxygen effect.