Production of fatty acids and derivatives thereof
View Patent ↗Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.
1. A method of producing a fatty alcohol composition, the method comprising culturing a recombinant bacteria in a medium containing a carbon source,
wherein the recombinant bacteria comprises exogenous nucleic acid sequences encoding:
(a) a polypeptide having acetyl-CoA carboxylase activity (EC 6.4.1.2);
(b) a polypeptide having acyl CoA synthase activity (EC 2.3.1.86);
(c) a polypeptide having thioesterase activity (EC 3.1.2.14); and
(d) a polypeptide selected from the group consisting of
(i) a fatty alcohol forming acyl-CoA reductase (FAR, EC 1.2.1.50);
(ii) an acyl-CoA reductase (EC 1.2.1.50); and
(iii) an alcohol dehydrogenase (EC 1.1.1.1).
2. The method of claim 1 , wherein said polypeptide (d) is alcohol forming acyl-CoA reductase (FAR, EC 1.2.1.50).
3. The method of claim 1 , wherein said fatty alcohol composition comprises one or more of saturated or unsaturated C12, C14, or C16 fatty alcohols.
4. The method of claim 1 , wherein the recombinant bacteria is E. coli.
5. A method of producing a branched fatty alcohol composition in a recombinant bacteria, the method comprising culturing the recombinant bacteria in a medium containing a carbon source, wherein the recombinant bacteria comprises:
(a) an exogenous nucleic acid sequence encoding a branched chain alpha-keto acid dehydrogenase 60 (Bkd) operon including a branched-chain α-keto acid decarboxylase α and β subunits (E1 α/β), a dihydrolipoyl transacylase component (E2), and a dihydrolipoyl dehydrogenase component (E3);
(b) an exogenous nucleic acid sequence encoding a β-ketoacyl-ACP synthase III protein (FabH, EC 2.3.1.41), having specificity for a branched chain acyl CoA molecule;
(c) a nucleic acid sequence encoding at least one polypeptide having thioesterase activity (EC 3.1.2.14); and
(d) an exogenous nucleic acid sequence encoding a polypeptide selected from the group consisting of:
(i) a fatty alcohol forming acyl-CoA reductase (FAR, EC 1.2.1.50),
(ii) an acyl-CoA reductase (EC 1.2.1.50); and
(iii) an alcohol dehydrogenase (EC 1.1.1.1).
6. The method of claim 5 , wherein said encoded polypeptide (d) is a fatty alcohol forming acyl-CoA reductase (FAR, EC 1.2.1.50).
7. The method of claim 5 , wherein the recombinant bacteria is E. coli.