Genetic loci associated with resistance of corn to fijivirus
The invention relates to methods and compositions for identifying and/or selecting maize plants that have newly conferred resistance or enhanced resistance to, or are susceptible to, a Fijivirus, particularly Mal de Rio Cuarto Virus (MRCV) and/or Maize Rough Dwarf Virus (MRDV). The methods use molecular genetic markers to identify, select, and/or construct resistant plants or to identify and counter-select susceptible plants. Maize plants that display newly conferred resistance or enhanced resistance to a Fijivirus (or an infection or disease caused by the virus) that are generated by the methods of the invention are also a feature of the invention.
1. A method of obtaining a maize plant with enhanced resistance to Mal de Rio Cuarto Virus (“MRCV”) infection, said method comprising:
a. isolating nucleic acids from a maize plant;
b. analyzing the isolated nucleic acids for the presence of a QTL allele associated with the enhanced resistance to MRCV, wherein the presence of said QTL allele is determined by detecting a haplotype within an interval on chromosome 4 comprising and flanked by PHM669 and PHM8008, wherein the reference sequence for PHM669 is SEQ ID NO:42 and the reference sequence for PHM8008 is SEQ ID NO:44, and said haplotype comprises: an “A” at position 85, a “C” at position 344, and an “A” at position 355 relative to SEQ ID NO:42;
c. selecting a maize plant having said QTL allele, based on the analysis of step b;
d. crossing said maize plant to a second maize plant;
e. evaluating progeny from said cross for the QTL allele; and
f. selecting progeny plants that possess the QTL allele.