Fluidic assay cartridge with controlled passive flow
A fluidic cartridge ( 110 ) with passively driven fluid flow is disclosed. The disclosed device and method solve problems associated with passive-flow fluidics by using a wicking pad ( 510 ) and/or a tilting means to prevent channel draining and backflow. The disclosure further provides means for adding liquid to the cartridge in multiple steps, without requiring liquid aspiration or removal.
1. A device for analyzing or processing a liquid sample, said device comprising a first substrate, a second substrate, a fluidic channel, an inlet port and an outlet port, said second substrate having an inner surface and an outer surface, said first substrate forming, at least in part, the lower wall of said fluidic channel, the inner surface of said second substrate forming, at least in part, the upper wall of said fluidic channel, said fluidic channel being connected to said inlet port and said outlet port, wherein said device further comprises a wicking pad being attached to the outer surface of said second substrate, said wicking pad being positioned at a distance from said outlet port, wherein said liquid sample has a top liquid surface height of h at the inlet port, said height h being defined relative to the outlet port, and relative to a level orientation.
2. The device of claim 1 , wherein said distance between the wicking pad and the outlet port is sufficient to prevent said wicking pad from draining the fluidic channel.
3. The device of claim 1 , wherein said distance between the wicking pad and the outlet port is sufficient to prevent liquid that has made contact with the wicking pad from flowing back into the outlet port.
4. The device of claim 1 , wherein said wicking pad is made of a material having a wicking rate of between 10 and 200 seconds per 4 centimeters (cm) of said material.
5. The device of claim 1 , further comprising a rail structure between the outlet port and the wicking pad for directing capillary flow from the outlet port to the wicking pad.
6. The device of claim 1 , wherein said distance between the wicking pad and the outlet port is between 1 and 5 mm.
7. The device of claim 1 , wherein said height h is achieved by placing said device in a rack with a pre-determined tilt angle.
8. The device of claim 1 , wherein said device is designed with an internal tilt relative to a level orientation, said internal tilt being sufficient to drive flow of said liquid sample from the inlet port to the outlet port.
9. The device of claim 8 , wherein said tilt is at an angle between 2 and 45 degrees relative to a level orientation.
10. The device of claim 1 , wherein said first substrate comprises a planar waveguide.
11. The device of claim 10 , further comprising a refractive volume for optically coupling to the planar waveguide a light beam provided by a light source.
12. The device of claim 11 , wherein said refractive volume is integrally formed from the planar waveguide.
13. A device for analyzing or processing a liquid sample, said device comprising a first substrate, a second substrate, a fluidic channel, an inlet port and an outlet port, said second substrate having an inner surface and an outer surface, said first substrate forming, at least in part, the lower wall of said fluidic channel, the inner surface of said second substrate forming, at least in part, the upper wall of said fluidic channel, said fluidic channel being connected to said inlet port and said outlet port wherein said device further comprises a wicking pad being attached to the outer surface of said second substrate, said wicking pad being positioned at a distance from said outlet port, wherein said liquid sample flows through the fluidic channel from the inlet port towards the outlet port after being added to the inlet port, wherein said liquid sample forms an inlet port reservoir at the vicinity of the inlet port before flowing through the fluidic channel, said inlet port reservoir having a depth of H 1 , H 1 being between 1 and 10 mm.
14. The device of claim 13 , wherein the flow of said liquid sample is driven by a pressure differential between the inlet port and the outlet port.
15. The device of claim 13 , wherein said wicking pad is made of a material having a wicking rate of between 10 and 200 seconds per 4 centimeters (cm) of said material.
16. The device of claim 13 , further comprising a rail structure between the outlet port and the wicking pad for directing capillary flow from the outlet port to the wicking pad.
17. The device of claim 13 , wherein said device is designed with an internal tilt relative to a level orientation, said internal tilt being sufficient to drive flow of said liquid sample from the inlet port to the outlet port.
18. The device of claim 13 , wherein said wicking pad is made of a material having a certain absorbance rate, wherein the surface tension of the liquid sample emerging from the outlet port breaks the fluidic connection between the wicking pad and the outlet port when said absorbance rate exceeds the rate at which said liquid sample emerges from the outlet port, thereby preventing further fluidic flow from the outlet port to the wicking pad.
19. The device of claim 18 , wherein said liquid flow is restored by adding liquid to the inlet port, without requiring removal of existing liquid from the device.