Method of calibrating and correcting color-bleed factors for color separation in DNA analysis

1. A method, comprising:

processing a color bleed calibration substance which includes a plurality of different size fragments replicated from different groups of DNA loci, wherein fragments in a same group are labeled with a same fluorescent dye, and fragments in different groups are labeled with different fluorescent dyes having different emission spectra, wherein the different size fragments are processed during different acquisition times;

illuminating the processed color bleed calibration substance with a light source;

detecting a fractional amount of signal emitted from a same fluorescent dye illuminated with the light source with a set of optical detector channels at an acquisition time, wherein the fractional amounts represent the color bleed factors of the optical detector channels for the dye;

producing, with the optical detectors, output signals that respectively include peak amplitudes indicative of the corresponding fractional amounts;

summing the amplitudes of the peaks;

determining a maximum height of the summed amplitudes;

determining a center of the peaks based on the maximum height;

determining a peak area for each optical channel based on the corresponding identified peak and a predefined acquisition time range around the corresponding peak center; and

calibrating an initial set of color bleed factors based thereon.

2. The method of claim 1 , further comprising: processing the color bleed calibration substance prior to processing a sample.

3. The method of claim 1 , further comprising: processing the color bleed calibration substance after processing a sample.

4. The method of claim 1 , further comprising: loading only the color bleed calibration substance prior to processing the color bleed calibration substance.

5. The method of claim 4 , further comprising:

generating a color bleed factor correction; and

correcting the initial set of color bleed factors with the correction.

6. The method of claim 1 , further comprising:

determining a total area for each detector channel; and

determining ratios of the total area of each detector channel to the total area of the detector channel corresponding to the emission spectrum of the emitted signal.

7. The method of claim 6 , further comprising:

calculating color-bleed factors within a particular locus by determining a total peak area based only on fragments within a fragment size range corresponding to the locus.

8. The method of claim 6 , further comprising:

calculating multiple sets of color-bleed factors in which each set includes only fragment peaks located within a certain range of fragment sizes, and the set is used for color separation of the signals acquired within that section of fragment size.

9. The method of claim 1 , further comprising:

generating, subsequently, a color bleed factor correction for the optical detector channels using the color bleed calibration substance at least one of before, during or after processing a DNA sample.

10. The method of claim 9 , further comprising:

correcting the set of relative color bleed factors based on the color bleed factor correction, wherein correcting includes one of scaling the relative color bleed factors or correcting the relative color bleed factors for changes in gain of the optical detectors channels.

11. The method of claim 10 , further comprising:

color separating an output signal, of the optical detector channels, indicative of processed DNA fragments using the corrected color bleed factors.

12. The method of claim 1 , wherein the color bleed calibration substance is included in one or more channels of a sample carrier inserted in and processed by the sample processing apparatus and is processed at least one of before or after processing a DNA sample with the sample processing apparatus.

13. The method of claim 1 , wherein the color bleed calibration substance is included in one or more channels of a sample carrier inserted in and processed by the sample processing apparatus.

14. The method of claim 1 , wherein at least one of the color bleed calibration substance is a positive control sample or a positive control sample is used as the color bleed calibration substance.

15. A sample processing system, comprising:

a sample carrier receptacle configured to receive a sample carrier carrying one or more samples to be processed by the sample processing system;

one or more processing stations for processing the one or more samples;

a reader, including an illumination source and one or more optical detector channels, that evaluates separated fragments of a processed sample based on emission spectrums of dyes attached to the fragments, and that generates an output signal;

a color separator that color separates a reader output signal corresponding to a processed DNA sample based on color bleed factors of the one or more optical detector channels; and

a color bleed factor generator and/or corrector configured to determine a color bleed factor correction for a set of color bleed factors for the optical detector channels based on processing a color bleed calibration substance, wherein the color bleed calibration substance includes a plurality of different size fragments in which different size fragments are grouped and labeled with different dye having different emission spectrums in different groups, and the different size fragments are processed and detected over different acquisition times,

wherein the color bleed calibration substance is processed separate from the DNA samples.

16. The sample processing system of claim 15 , wherein the color bleed factor generator and/or corrector corrects the set of color bleed factors based on the color bleed factor correction.

17. The sample processing system of claim 16 , wherein the correction corresponds to a change in gain of the optical detector channels.

18. The sample processing system of claim 15 , wherein the color bleed calibration substance is processed prior to processing the DNA samples.

19. The sample processing system of claim 15 , wherein the color bleed factor generator and/or corrector sums amplitudes of peaks of all the channels at an acquisition time, determines a maximum height of the summed amplitudes, determines a center of the peaks based on the maximum height, determines a peak area for each optical channel based on the corresponding identified peak and a predefined acquisition time ran e around the corresponding peak center, and determine the color bleed factors based thereon.