Lung Cancer Tests
Methods for the diagnosis of lung cancer and more specifically, non-small cell lung cancer are described. An assortment of biomarkers with diagnostic or prognostic value for non-small cell lung cancer (NSCLC) are used to establish a multi-analyte serum test capable of diagnosing patients with lung cancer. A first method assesses an elevated level of expression of one or more nucleic acids, a polypeptide encoded by the nucleic acid or an autoantibody to said polypeptide. An alternative method includes (a) determining whether or not a patient has NSCLC versus a non-NSCLC lung cancer, and (b) classifying the patient as susceptible to specific treatments if the patient has a NSCLC profile and classifying the patient as not susceptible to a specific treatment if the patient does not have a NSCLC profile.
1 . A method of assessing non-small cell lung cancer (NSCLC) of a mammal, the method comprising:
collecting a biological sample from the mammal; and
measuring whether the mammal has an expression of at least one of a nucleic acid, at least one polypeptide encoded by said nucleic acid and at least one autoantibody to said polypeptide;
wherein a presence or absence of said nucleic acid, said polypeptide encoded by said nucleic acid and said autoantibody to said peptide is indicative that the mammal has NSCLC.
2 . The method of claim 1 further comprising:
using the presence or absence of said nucleic acid, said polypeptide encoded by said nucleic acid and said autoantibody to said peptide to determine a treatment for NSCLC.
3 . The method of claim 1 wherein said nucleic acid, said polypeptide encoded by said nucleic acid and said autoantibody to said polypeptide are selected from the group consisting of TNF-α, CYFRA 21.1, IL-1ra, IL-6, IFN-γ, IL-2Rα, CA125, MCP-1, CRP, MMP-2 and sE-selectin.
4 . The method of claim 1 wherein the biological sample is selected from the group consisting of lung tissue, sputum and blood.
5 . The method of claim 1 wherein the measuring further comprises:
detecting an elevated level of one or more of said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide in the biological sample, and
wherein said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide are selected from the group consisting of TNF-α, CYFRA 21.1, IL-1ra, MCP-1, MMP-2 and sE-selectin.
6 . The method of claim 1 wherein the measuring comprises:
determining a presence or an absence of one or more of said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide in the biological sample,
wherein said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide are selected from the group consisting of TNF-α, CYFRA 21.1, IL-1ra, MCP-1, MMP-2 and sE-selectin, and
the method further comprises selecting a treatment for NSCLC based on the presence or absence of said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide in the biological sample.
7 . The method of claim 1 further comprising detecting elevated levels of a plurality of nucleic acids simultaneously using a nucleic acid array.
8 . The method of claim 1 further comprising detecting elevated levels of a plurality of polypeptides simultaneously using a polypeptide array.
9 . The method of claim 1 further comprising using the presence or absence of said nucleic acid, said polypeptide encoded by said nucleic acid and said autoantibody to said peptide to assess NSCLC risk and determining whether the mammal requires further screening with computed tomography (CT).
10 . The method of claim 1 wherein the level can be determined using at least one of PCR, in situ hybridization or immunohistochemistry.
11 . The method of claim 1 further comprising:
determining whether or not the mammal has lung cancer versus a nonmalignant disease by detecting an elevated level of said nucleic acid, said polypeptide encoded with said nucleic acid and said autoantibody to said polypeptide is selected from the group consisting of IMPDH, phosphoglycerate mutase, ubiquillin, annexin I, annexin II, and heat shock protein 70-9B (HSP70-9B).
12 . The method of claim 1 further comprising:
determining whether or not a mammal having lung cancer versus a nonmalignant disease by determining the level of said nucleic acid, said polypeptide encode with said nucleic acid and said autoantibody to said polypeptide is selected from the group consisting of inosine-5-monophosphate dehydrogenase (IMPDH), fumarate hydratase (FH), α-enolase, endoplasmic reticulum protein 29 (Erp29), annexin I, hydrosteroid 17-[3 dehydrogenase, methylthioadenosine phosphorylase (MTAP), annexin II, ubiquilin, c-Myc, NY-ESO, 3-oxoacid CoA transferase, p53 phosphoglycerate mutase, and heat shock protein 70-9B (HSP70-9B)]; and
using the presence or absence of an elevated level to determine treatment for a lung cancer such as NSCLC.