Compositions of toehold primer duplexes and methods of use
View Patent ↗Provided herein are primers and primer systems having improved specificity and kinetics over existing primers, and methods of use thereof.
1. A partially double-stranded primer comprised of first and second nucleic acid strands arranged into
(1) one double-stranded target-non-specific region,
(2) one double-stranded target-specific region, and
(3) one single-stranded target-specific region contributed to by the first nucleic acid strand,
wherein the double-stranded target-non-specific region has a standard free energy that is within 10% of the standard free energy for the single-stranded target-specific region bound to a perfectly complementary region of a target nucleic acid, wherein the sequence of the first nucleic acid strand that contributes to region (1) is not perfectly complementary to and does not bind to the target nucleic acid, and wherein the sequences of regions (2) and (3) are complementary to and bind to the target nucleic acid.
2. The partially double-stranded primer of claim 1 , wherein the second nucleic acid strand comprises a non-extendable nucleotide at its 3′ end and/or the first nucleic acid strand comprises a non-natural nucleotide at or near the 3′ end of its target-non-specific region.
3. The partially double-stranded primer of claim 2 , wherein the non-extendable nucleotide is a non-natural nucleotide or a dideoxy nucleotide.
4. The partially double-stranded primer of claim 3 , wherein the non-natural nucleotide is iso-C, iso-G or deoxyuridine.
5. The partially double-stranded primer of claim 1 , wherein the double-stranded target non-specific region is about 4-21 nucleotides in length.
6. The partially double-stranded primer of claim 1 , wherein the single-stranded target-specific region is about 4-20 nucleotides in length.
7. The partially double-stranded primer of claim 1 , wherein the first and second nucleic acid strands are comprised of DNA or RNA.
8. A system comprising
a nucleic acid target,
a polymerase, and
a partially double-stranded primer comprising a first and a second nucleic acid strand arranged into
(1) one double-stranded target-non-specific region,
(2) one double-stranded target-specific region, and
(3) one single-stranded target-specific region contributed to by the first nucleic acid strand,
wherein the double-stranded target-non-specific region has a standard free energy that is within 10% of the standard free energy for the single-stranded target-specific region bound to a perfectly complementary region of a target nucleic acid, wherein the sequence of the first nucleic acid strand that contributes to region (1) is not perfectly complementary to and does not bind to the target nucleic acid, and wherein the sequences of regions (2) and (3) are complementary to and bind to the target nucleic acid.
9. The system of claim 8 , wherein the nucleic acid target is a single-stranded.
10. The system of claim 8 , wherein the nucleic acid target is DNA or RNA.
11. The system of claim 8 , wherein the system comprises a plurality of different partially double-stranded primers.
12. The system of claim 8 , wherein the system comprises at least two partially double-stranded primers which together can be used to amplify a region of the nucleic acid target.
13. The system of claim 8 , wherein the nucleic acid target comprises repetitive sequence, secondary structure and/or high GC content.
14. A method comprising
contacting a partially double-stranded primer of claim 1 to a sample, and detecting hybridization of the primer to a target in the sample.
15. A method comprising
hybridizing a single-stranded target-specific region of a first strand of a partially double-stranded primer of claim 1 to a nucleic acid target, thereby dissociating the first strand of the primer from a second strand of the primer, and
extending the first strand at its 3′ end, in a target-complementary manner, in the presence of a polymerase.
16. A method comprising
performing a nucleic acid synthesis reaction in the presence of a nucleic acid target, a polymerase, and one or more partially double-stranded primers of claim 1 .
17. A kit comprising
one or more partially double-stranded primers of claim 1 , and
one or more nucleic acid synthesis reagents.
18. A kit comprising
a first single-stranded nucleic acid in a first container, and
a second single-stranded nucleic acid that is complementary to a region of the first single-stranded nucleic acid, in a second container,
wherein, when the first and second single-stranded nucleic acids are hybridized to each other, a partially double-stranded nucleic acid is formed that comprises
(1) a double-stranded target-non-specific region,
(2) a double-stranded target-specific region, and
(3) a single-stranded target-specific region contributed to by the first nucleic acid,
wherein the double-stranded target-non-specific region has a standard free energy that is within 10% of the standard free energy for the single-stranded target-specific region bound to a perfectly complementary region of a target nucleic acid, wherein the sequence of the first nucleic acid strand that contributes to region (1) is not perfectly complementary to and does not bind to the target nucleic acid, and wherein the sequences of regions (2) and (3) are complementary to and bind to the target nucleic acid.
19. The partially double-stranded primer of claim 1 , wherein the standard free energy of regions (2) and (3) bound to the target is greater than −3.5 kcal/mol and less than 3.5 kcal/mol.
20. The partially double-stranded primer of claim 1 , wherein the standard free energy of regions (2) and (3) bound to the target is greater than −1 kcal/mol and less than 1kcal/mol.