Protein arrays and methods of using and making the same
Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.
1. A method for preparing a protein array having a plurality of proteins, the method comprising:
(a) providing a plurality of nucleic acids each having a predefined sequence and each encoding a protein of interest, wherein the plurality of nucleic acids are immobilized on discrete features of a surface; and
(b) expressing in vitro a plurality of proteins of interest from the plurality of nucleic acids, wherein the plurality of proteins are each present in solution at the discrete features on the surface;
wherein each of the plurality of proteins of interest is expressed in a self-contained volume having a size of 1 picoliter to 1 nanoliter.
2. The method of claim 1 , further comprising (c) measuring an activity of each of the plurality of proteins that are present in solution.
3. The method of claim 1 , wherein the plurality of nucleic acids are synthesized on a solid surface.
4. The method of claim 1 , wherein each of the plurality of nucleic acids comprises a regulatory genetic sequence.
5. The method of claim 1 , wherein each of the plurality of proteins of interest is expressed in vitro in a well of a micro-well plate.
6. The method of claim 1 , wherein each of the plurality of proteins of interest is expressed in vitro at a different feature of a solid surface.