L-amino-acid-containing feed additive in the form of fermentation-broth-based granules, and processes for its preparation
The invention relates to a granular fermentation-broth-based feed additive, comprising an L-amino acid selected from the group consisting of L-lysine, L-methionine, L-threonine, L-tryptophan, L-valine or mixtures of these, suitable for the preparation of feeds for animals kept in aquaculture, where the content of the L-amino acid or the mixtures thereof amount to 20% by weight of the total composition of the granular feed additive; where at least 70% by weight of the particles have a particle diameter of from >63 μm to <300 μm, where the biomass content amounts to ≧5% by weight and where the surface of the particles is fully or partially coated with an edible oil, wherein the L-amino acid is L-lysine, is present as a sulphate salt and the molar ratio of sulphate to L-lysine amounts to at least 0.5.
1 . A granular fermentation-broth-based feed additive, comprising an L-amino acid selected from the group consisting of: L-lysine, L-methionine, L-threonine, L-tryptophan, and L-valine, or mixtures thereof, suitable for the preparation of feeds for animals kept in aquaculture, and wherein:
a) the content of said L-amino acid or mixture of L-amino acids comprises ≧20% by weight of the total weight of the feed additive;
b) the feed additive comprises particles and at least 70% by weight of said particles have a particle diameter of from >63 μm to <300 μm;
c) the feed additive has a biomass content of ≧5% by weight and
d) the surface of the particles in said granular feed additive is fully or partially coated with an edible oil.
2 . The feed additive of claim 1 wherein said L-amino acid is L-lysine present as a sulphate salt and wherein the molar ratio of sulphate to L-lysine is at least 0.5.
3 . The feed additive of claim 1 , comprising biomass derived from the genus Corynebacterium or the genus Escherichia.
4 . The feed additive of claim 1 , wherein the content of said L-amino acid or mixture of L-amino acids is ≧30% by weight of the total weight of the feed additive.
5 . The feed additive of claim 1 , wherein the content of said L-amino acid or mixture of L-amino acids is ≧60% by weight of the total weight of the feed additive.
6 . The feed additive claim 1 wherein at least 75% by weight of the particles have a particle size diameter of from >63 μm to <300 μm.
7 . The feed additive claim 1 wherein at least 75% by weight of the particles have a particle size diameter of from >63 μm to <280 μm.
8 . The feed additive of claim 1 , comprising a biomass content of ≧10% by weight.
9 . The feed additive of claim 1 , comprising a biomass content of ≧50% by weight.
10 . The feed additive of claim 1 , comprising a biomass content of ≧80% by weight.
11 . The feed additive of claim 1 , wherein the edible oil is selected from the group consisting of: a vegetable oil, an animal oil or fat, or an oil produced fermentatively by a microorganism.
12 . The feed additive of claim 1 , wherein the edible oil is a vegetable oil selected from the group consisting of: soybean oil, olive oil, and sunflower seed oil.
13 . The feed additive of claim 1 , wherein the edible oil accounts for 0.01 to 2% by weight of the total feed additive composition.
14 . The feed additive of claim 1 , wherein at least 70% by weight of the particles have a particle size diameter of from >63 μm to <300 μm, with the particle size distribution being measured by screening analysis in a Hosokawa Alpine air-jet screening apparatus, type 200 LS-N, screening set: mesh sizes 20, 32, 45, 63, 100, 150, 200, 250, 280, 300, 400, 500, 600, 630, 710, 800, 1000, 1180, 1400, 1600 and 2000 μm; using a screening time of 3 minutes.
15 . The feed additive of claim 3 wherein said L-amino acid is L-lysine present as a sulphate salt and wherein the molar ratio of sulphate to L-lysine is at least 0.5.
16 . The feed additive of claim 15 , wherein:
a) at least 75% by weight of the particles have a particle size diameter of from >63 μm to <280 μm; and
b) the content of said L-amino acid is ≧60% by weight of the total weight of the feed additive.
17 . The feed additive of claim 16 , wherein:
a) said feed additive comprises a biomass content of ≧80% by weight;
b) the edible oil in said feed additive is selected from vegetable oil, animal oil or fat, or oil produced fermentatively by microorganisms; and
c) the edible oil accounts for 0.01 to 2% by weight of the total feed additive composition.
18 . A process for the preparation of the feed additive of claim 1 , comprising the steps:
a) fermenting an L-amino-acid-producing microorganism in an aqueous nutrient medium under aerobic conditions, wherein said L-amino acid is selected from the group consisting of L-lysine, L-methionine, L-threonine, L-tryptophan, and L-valine;
b) concentrating the fermentation broth by evaporation;
c) in the event that the L-amino acid is L-lysine: adding of ammonium sulphate and/or sulphuric acid, wherein a sulphate/L-amino acid ratio of from 0.85 to 1.2 in the broth is established;
d) removing water from the mixture and drying to give granules;
e) adjusting particle size to give a product which contains at least 70% by weight of particles with a particle size of from >63 μm to <300 μm; and
f) coating the particles of step d) and/or e) with an edible oil to give particles which are fully or partially coated by the edible oil.
19 . A feed for animals kept in aquaculture, comprising the additive of claim 1 .