METHOD OF MAKING A PAIRED TAG LIBRARY FOR NUCLEIC ACID SEQUENCING
The present disclosure relates to methods and compositions for making paired tags and paired tag libraries.
1 - 91 . (canceled)
92 . A plurality of circular nucleic acid molecules, the individual circular molecules in the plurality comprising:
(a) a universal sequence region common to the plurality of circular nucleic acid molecules and having two nicks; and
(b) a target sequence region that differs between different molecules of the plurality of circular molecules.
93 . The molecules of claim 92 , wherein the universal sequence region comprises a double-stranded nucleic acid.
94 . The molecules of claim 92 , wherein the universal sequence further comprises a binding moiety.
95 . The molecule of claim 94 , wherein the binding moiety comprises biotin.
96 . The molecules of claim 94 , further comprising a purifying moiety.
97 . The molecules of claim 96 wherein the purifying moiety comprises streptavidin.
98 . The molecule of claim 92 , further comprising a nick translating enzyme.
99 . The molecule of claim 98 , wherein the nick translating enzyme comprises DNA polymerase I.
100 . A plurality of circular nucleic acid molecules, the individual circular molecules in the plurality comprising:
(a) a universal sequence region common to the plurality of circular nucleic acid molecules, the universal sequence region having a first end and a second end;
(b) a target sequence region that differs between different molecules of the plurality of circular molecules, the target sequence region having a first end and a second end;
(c) a first nick between the first end of the universal sequence region and the first end of the target sequence region; and
(d) a second nick between the second end of the universal sequence region and the second end of the target sequence region.