Extraction solvents derived from oil for alcohol removal in extractive fermentation
View Patent ↗In an alcohol fermentation process, oil derived from biomass is hydrolyzed into an extractant available for in situ removal of a product alcohol such as butanol from a fermentation broth. The glycerides in the oil can be catalytically (e.g., enzymatically) hydrolyzed into free fatty acids, which form a fermentation product extractant having a partition coefficient for a product alcohol greater than a partition coefficient of the oil of the biomass for the product alcohol. Oil derived from a feedstock of an alcohol fermentation process can be hydrolyzed by contacting the feedstock including the oil with one or more enzymes whereby at least a portion of the oil is hydrolyzed into free fatty acids forming a fermentation product extractant, or the oil can be separated from the feedstock prior to the feedstock being fed to a fermentation vessel, and the separated oil can be contacted with the enzymes to form the fermentation product extractant. The fermentation product extractant can be contacted with a fermentation broth for in situ removal of a product alcohol.
1. A composition comprising:
(a) a recombinant microorganism capable of producing butanol;
(b) fermentable carbon source selected from monosaccharides, disaccharides, oligosaccharides, polysaccharides, C5 sugars, and mixtures thereof;
(c) at least one enzyme capable of hydrolyzing glycerides into fatty acids;
(d) oil comprising glycerides; and
(e) fatty acids.
2. The composition of claim 1 , wherein the at least one enzyme is esterase.
3. The composition of claim 1 , wherein the oil is corn, tallow, canola, capric/caprylic triglycerides, castor, coconut, cottonseed, fish, jojoba, lard, linseed, neetsfoot, oiticica, palm, peanut, rapeseed, rice, safflower, soya, sunflower, tung, jatropha and vegetable oil blends.
4. The composition of claim 1 , wherein the fermentable carbon source and the oil are derived from biomass.
5. The composition of claim 4 , wherein the biomass comprises corn grain, corn cobs, corn husks, corn stover, wheat, rye, wheat straw, barley, barley straw, hay, rice straw, switchgrass, sugar cane bagasse, sorghum, sugar cane, soy, and mixtures thereof.
6. The composition of claim 1 , further comprising a saccharification enzyme.
7. The composition of claim 1 , further comprising undissolved solids.
8. The composition of claim 1 , further comprising glycerol and/or butanol.
9. The composition of claim 8 , wherein the butanol is 1-butanol, 2-butanol, isobutanol, or mixtures thereof.
10. The composition of claim 2 , wherein the esterase is selected from lipase, phospholipase, and lysophospholipase.
11. The composition of claim 1 , wherein the recombinant microorganism comprises a butanol biosynthetic pathway.
12. The composition of claim 11 , wherein the recombinant microorganism comprises an isobutanol biosynthetic pathway.
13. The composition of claim 12 , wherein the recombinant microorganism comprises a reduction or elimination of pyruvate decarboxylase activity.
14. The composition of claim 1 , wherein the recombinant microorganism is yeast.
15. The composition of claim 14 , wherein the yeast is Saccharomyces cerevisiae.