DIG-10 insecticidal cry toxins
View Patent ↗DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.
1. A transgenic plant comprising a polynucleotide comprising SEQ ID NO:1 or SEQ ID NO:3 operably linked to a promoter that is not derived from Bacillus thuringiensis.
2. A transgenic plant comprising a polypeptide comprising SEQ ID NO:5.
3. The plant of claim 2 , wherein the polypeptide has insecticidal activity against corn rootworm.
4. A method for controlling a pest population comprising contacting said population with a pesticidally effective amount of a polypeptide comprising SEQ ID NO:5.
5. A DNA construct comprising a nucleic acid encoding a polypeptide comprising SEQ ID NO:2 or SEQ ID NO:5 or an insecticidally active fragment of SEQ ID NO:2 or SEQ ID NO:5 operably linked to a promoter that is not derived from Bacillus thuringiensis and is capable of driving expression in a plant.
6. A transgenic plant that comprises a nucleic acid encoding a polypeptide comprising SEQ ID NO:2 or SEQ ID NO:5 or an insecticidally active fragment thereof of SEQ ID NO:2 or SEQ ID NO:5 operably linked to a promoter that is not derived from Bacillus thuringiensis stably incorporated into its genome.
7. A method for protecting a plant from a pest comprising introducing into said plant the construct of claim 5 .
8. The transgenic plant of claim 6 wherein said transgenic plant comprises a dsRNA for suppression of an essential gene in an insect pest.
9. The transgenic plant of claim 6 wherein said transgenic plant comprises a dsRNA for suppression of an essential gene in corn rootworm.
10. The transgenic plant of claim 9 wherein said essential gene is selected from the group consisting of vacuolar ATPase, ADP-ribosylation factor-1, actin 42A, beta-tubulin CHD3, elongation factor-1.alpha., and transcription factor IIB.
11. The DNA construct of claim 5 wherein said DNA construct further comprises a gene encoding a dsRNA for suppression of an essential gene in an insect pest.
12. The transgenic plant of claim 6 wherein a gene encoding a dsRNA for suppression of an essential gene in an insect pest is stably incorporated into its genome.