Method of detecting a urine protein fragment and a serum protein fragment
Described is a method of detecting a protein fragment in a urine sample and a serum sample. The protein fragment is a urine precursor alpha-2-HS-glycoprotein, urine alpha-1 antitrypsin, urine alpha-1 acid glycoprotein, urine osteopontin, or serum osteopontin fragment. The urine sample and the serum sample are obtained from the same individual.
1. A method of detecting a protein fragment, comprising:
(i) detecting in a urine sample the level of a urine protein fragment selected from the group consisting of VVSLGSPSGEVSHPRKT (SEQ ID NO:1), MGVVSLGSPSGEVSHPRKT (SEQ ID NO:2), KGKWERPFEVKDTEEEDF (SEQ ID NO:3), EDPQGDAAQKTDTSHHDQDHPTFNKITPNLAE (SEQ ID NO:5), EDPQGDAAQKTDTSHHDQDHPTFNKITPNLAEFA (SEQ ID NO:6), YPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSK (SEQ ID NO:8) or KYPDAVATWLNPDPSQKQNLLAPQTLPSK (SEQ ID NO:9); and
(ii) detecting in a serum sample the level of a serum protein fragment selected from the group consisting of YPDAVATWLNPDPSQKQNLLAPQNAVSSEETNDFKQETLPSK (SEQ ID NO:8) and KYPDAVATWLNPDPSQKQNLLAPQTLPSK (SEQ ID NO:9), wherein if detecting SEQ ID NO:8 is performed in the serum sample, detecting SEQ ID NO:8 is not performed in the urine sample, and if detecting SEQ ID NO:9 is performed in the serum sample, detecting SEQ ID NO:9 is not performed in the urine sample, the urine sample and the serum sample being obtained from the same subject.
2. The method of claim 1 , wherein the level of a urine protein fragment selected from the group consisting of SEQ ID NOs:3, 5, and 6 is detected in the urine sample.
3. The method of claim 2 , wherein the urine protein fragment detected is SEQ ID NO:3.
4. The method of claim 2 , wherein the urine protein fragment detected is SEQ ID NO:5.
5. The method of claim 2 , wherein the urine protein fragment detected is SEQ ID NO:6.
6. The method of claim 2 , further comprising detecting the level of a different urine protein fragment selected from the group consisting of SEQ ID NO:1, 2, and 7 in the urine sample.
7. The method of claim 6 , wherein the different urine protein fragment is SEQ ID NO:1 or 2.
8. The method of claim 7 , further comprising detecting in the urine sample the level of SEQ ID NO:7.
9. The method of claim 8 , further comprising detecting in the serum sample the level of SEQ ID NO:8.
10. The method of claim 8 , further comprising detecting in the urine sample the level of SEQ ID NO: 8.
11. The method of claim 6 , wherein the different urine protein fragment is SEQ ID NO:7.
12. The method of claim 1 , wherein detecting steps (i) and (ii) are both performed with an immune assay.
13. The method of claim 1 , wherein the level of a urine protein fragment selected from the group consisting of SEQ ID NOs:1, 2, 3, 5, and 6 is detected in the urine sample.
14. The method of claim 13 , further comprising detecting in the urine sample the level of SEQ ID NO:7.
15. The method of claim 1 , wherein the level of a urine protein fragment selected from the group consisting of SEQ ID NOs:1, 2, 3, 5, 6, and 8 is detected in the urine sample and the level of serum protein fragment SEQ ID NO: 9 is detected in the serum sample.
16. The method of claim 15 , further comprising detecting in the urine sample the level of SEQ ID NO:7.
17. The method of claim 1 , wherein the level of a urine protein fragment selected from the group consisting of SEQ ID NOs:1, 2, 3, 5, 6, and 9 is detected in the urine sample and the level of serum protein fragment SEQ ID NO: 8 is detected in the serum sample.
18. The method of claim 17 , further comprising detecting in the urine sample the level of SEQ ID NO:7.