Adapters for ligation to RNA in an RNA library with reduced bias
View Patent ↗An improved adapter design for ligation to target RNA in a library is described which enables target RNAs that would normally be under-represented or not represented at all in a cDNA library or a PCR product of the cDNA library, to be represented with average frequency.
1. A set of reagents for producing adaptor-ligated RNA, comprising:
(a) a population of 3′ polynucleotide adaptors each having a 3′ end and a 5′ end,
wherein each adaptor comprises:
(i) a first non-random sequence of at least seven defined nucleotides at the 5′ end,
(ii) a first random nucleotide sequence located between the 3′ end and the first defined nucleotide sequence,
(iii) a third non-random sequence of at least 5 defined nucleotides, and
(iv) a modified nucleotide at the 3′ end,
and wherein the first random nucleotide sequence varies in the population of 3′ polynucleotide adaptors;
(b) a 5′ polynucleotide adapter, the 5′ adapter having a 3′ end and a 5′ end and comprising a second non-random sequence of at least seven contiguous nucleotides that is complementary to the first non-random sequence of (a)(i); and
(c) a ligase capable of ligating RNA.
2. The set of reagents according to claim 1 , wherein the length of the first random nucleotide sequence is in the range of 2 nucleotides to 50 nucleotides.
3. The set of reagents according to claim 1 , wherein the 3′ polynucleotide adapter further comprises a barcode sequence.
4. The set of reagents according to claim 1 , wherein the 3′ polynucleotide adaptor is a DNA oligonucleotide and the 5′ polynucleotide adapter is an RNA oligonucleotide.
5. A method of ligating adapters to target RNA having 3′ and 5′ ends in an RNA library, comprising:
obtaining the set of reagents of claim 1 ;
ligating the 3′ adapter to an RNA in an RNA library;
allowing a portion of the 5′ adapter to hybridize with a portion of the sequence of the 3′ adapter; and
ligating the hybridized 5′ end RNA adapter to the RNA.
6. The set of reagents of claim 1 , wherein:
(a) the 3′ adapter comprises, from 5′ to 3′: the first non-random sequence, the first random nucleotide sequence, and the third non-random sequence; and
(b) the 5′ adaptor comprises a second random nucleotide sequence that is 5′ to the second non-random sequence of (b).
7. The set of reagents of claim 6 , wherein the first and second random nucleotide sequences are independently in the range of 2 to 50 nucleotides in length.
8. The set of reagents of claim 6 , wherein the 3′ adapter is 5′ adenylated.
9. The set of reagents of claim 6 , wherein the 5′ adaptor comprises a 5′ hydroxyl.
10. The set of reagents of claim 6 , wherein the 3′ and 5′ adaptors are both DNA oligonucleotides.
11. A set of reagents according to claim 1 , wherein the wherein the 3′ and 5′ adaptors are both RNA oligonucleotides.
12. The set of reagents of claim 6 , wherein the ligase is a T4 RNA ligase.
13. A set of reagents, comprising:
(a) a population of 3′ polynucleotide adaptors each having a 3′ end and a 5′ end wherein each adapter, comprises:
(i) a first non-random sequence of at least seven defined nucleotides at the 5′ end,
(ii) a first random nucleotide sequence located between the 3′ end and the first defined nucleotide sequence, wherein each of the first random sequences in the population of 3′ adapters comprises a different sequence
(iii) a third non-random sequence of at least 5 defined nucleotides, and
(iv) a modified nucleotide at the 3′ end, and wherein the first random nucleotide sequence varies in the population of 3′ polynucleotide adaptors;
(b) a 5′ polynucleotide adapter, the 5′ adapter having a 3′ end and a 5′ end and comprising a second non-random sequence of at least seven contiguous nucleotides that is complementary to the first non-random sequence of (a)(i);
(c) a ligase capable of ligating RNA;
(d) a reverse transcription primer comprising a 3′ end that is complementary with and hybridizes to the third non-random sequence of (a)(iii); and
(e) a reverse transcriptase.
14. The set of reagents of claim 6 , wherein the 3′ adapter further comprises a barcode.
15. The set of reagents of claim 6 , further comprising a reverse transcriptase.
16. The set of reagents of claim 6 , wherein the 5′ and 3′ adaptors contain a primer binding site in the first or second defined nucleotide sequence or in a third or fourth defined nucleotide sequence.
17. A method of ligating adapters to target RNA having 3′ and 5′ ends in an RNA pool, comprising:
obtaining the set of reagents of claim 6 ;
ligating the 3′ adapter to an RNA in a pool of RNA;
allowing the 3′ end of the 5′ adapter to hybridize with the 5′ end of the 3′ adapter; and
ligating the hybridized 3′ end of the 5′ adapter to the RNA.