Fusion polypeptides comprising an active protein linked to a mucin-domain polypeptide
View Patent ↗The present invention provides fusion proteins comprising a mucin-domain polypeptide covalently linked to an active protein that has improved properties (e.g. pharmacokinetic and/or physicochemical properties) compared to the same active protein not linked to mucin-domain polypeptide, as well as methods for making and using the fusion proteins of the invention.
1. A fusion protein comprising a mucin-domain polypeptide linked to at least one active protein wherein the mucin-domain polypeptide has at least 4 tandem amino acid repeating units of at least 8 amino acids in length per tandem repeating unit, wherein the active protein is exendin-4; and wherein the half-life of the fusion protein is increased by two fold as compared to the half-life of the corresponding active protein that is not fused to the mucin-domain polypeptide, and wherein the fusion protein comprises the amino acid sequence of SEQ ID NO: 24.