Use of a fluorescent material to detect failure or deteriorated performance of a fluorometer
A System and method for self-checking a fluorometer for failure or deteriorated performance includes fluorescent reference standards mounted on a support to move with respect to one or more fixed fluorometers. The intensity of the fluorescent emission of the fluorescent reference standard is initially measured with the fluorometer, and, after a prescribed interval of usage of the fluorometer, a test measurement of the intensity of the fluorescent emission of the fluorescent standard is taken with the fluorometer. The test measurement is compared to the initial measurement, and failure or deteriorated performance of the fluorometer is determined based on a deviation of the test measurement from the initial measurement.
1. A system for monitoring the performance of a fluorometer in a dynamic environment, comprising
a fluorometer comprising two or more channels, each channel having a separate light source, optical focus and filter assembly, and optical signal detector, and wherein each channel is configured to focus the light source at a detection zone;
a support comprising two or more fluorescent reference standards, each fluorescent reference standard corresponding to a single channel of the fluorometer, wherein the support is arranged to accommodate two or more removable receptacle vessels; and
a drive mechanism configured to adjust the relative horizontal positioning between the reference standards and the fluorometer such that each of the two or more fluorescent reference standards can be positioned in or out of optical communication with its corresponding channel of the fluorometer.
2. The system of claim 1 , wherein the fluorescent reference standard is positioned in optical communication with the corresponding channel of the fluorometer, and wherein the fluorescent reference standard is out of focus relative to the detection zone.
3. The system of claim 1 , wherein the two or more fluorescent reference standards are positioned in a linear arrangement on the support.
4. The system of claim 3 , wherein at least one of the fluorescent reference standards in the linear arrangement has emission characteristics that differ from at least one of the other fluorescent reference standards in the linear arrangement.
5. The system of claim 1 , wherein only one of the fluorescent reference standards can be positioned in optical communication with one of the two or more channels of the fluorometer at a time.
6. The system of claim 2 , wherein the fluorescent reference standard is positioned between about 1% to 99% closer to the corresponding channel relative to the detection zone.
7. The system of claim 2 , wherein the fluorescent reference standard is positioned at the same distance from its corresponding channel as the distance between the channel and the detection zone.
8. The system of claim 2 , wherein the fluorescent reference standard is positioned between about 1% to 99% farther from its corresponding channel relative to the detection zone.
9. The system of claim 1 , wherein the fluorometer is stationary, and the drive mechanism is configured to adjust the relative horizontal positioning between the reference standards and the fluorometer by adjusting the horizontal positioning of the support.
10. The system of claim 1 , wherein the support comprises a rotatable carousel, and wherein the fluorometer is fixed with respect to the carousel and the drive mechanism is configured to adjust the relative horizontal positioning between the reference standards and the fluorometer by effecting angular movement of the carousel around a central axis.
11. The system of claim 1 , wherein the system comprises two or more fluorometers, each fluorometer comprising two or more channels, each channel having a separate light source, optical focus and filter assembly, and optical signal detector, and wherein each channel is configured to focus the light source at a detection zone.
12. The system of claim 11 , wherein each fluorometer has a different light source, optical focus and filter assembly, and optical signal detector, such that each fluorometer emits a different excitation signal and detects a different emission signal.