Color-based reaction testing of biological materials
A biological material test strip and adjacently-located reference color chart are affixed to a lid portion of an all-in-one specimen cup to perform color-based reaction testing of collected biological specimens in an uncalibrated environment. After specimen collection, the lid portion is secured to a container portion of the specimen cup. The cup may then be rotated into an upside down position causing the specimen, under the force of gravity, to pass from the container portion and into a volume of the lid portion, such that the test strip is exposed to the specimen as it is received into the volume of the lid portion. An image of the exposed test strip and adjacently-located reference color chart may then be captured and processed to identify any color matches between the individual test pads on the test strip and the corresponding sequences of reference color blocks on the reference chart.
1. A method for color-based reaction testing of biological materials comprising the acts of:
capturing, by an electronic device using ambient lighting in an uncalibrated environment, digital image information of an exposed test strip and of a reference color chart, wherein the digital image information of each of the exposed test strip and the reference color chart is captured in the same uncalibrated environment, wherein the exposed test strip comprises a plurality of test pads, and wherein further the reference color chart comprises a plurality of color blocks;
locating, by a diagnostic software application that is executing on the electronic device, first image data within the digital image information corresponding to each of the plurality of test pads, said first image data having uncalibrated color information;
locating, by the diagnostic software application executing on the electronic device, second image data within the digital image corresponding to the plurality of color blocks on the reference color chart, said second image data having uncalibrated color information;
matching, by the diagnostic software application executing on the electronic device, uncalibrated color information from the first image data to corresponding uncalibrated color information from the second image data, wherein said matching comprises comparing uncalibrated color information corresponding to a first test pad of the plurality of test pads to uncalibrated color information from at least one of the plurality of color blocks on the reference color chart;
generating, by the diagnostic software application executing on the electronic device, test results based on said matching; and
displaying, on a display screen of the electronic device, a graphical representation of the test results indicating an absence, presence and degree of presence of a reactant within the biological specimen.
2. The method of claim 1 , wherein locating first image data comprises applying, by the diagnostic software application executing on the electronic device, one of a shape location algorithm and a template matching operation to the first image data.
3. The method of claim 2 , wherein locating second image data comprises applying, by the diagnostic software application executing on the electronic device, one of the shape location algorithm and the template matching operation to the second image data.
4. The method of claim 1 , further comprising averaging, by the diagnostic software application executing on the electronic device, color information across the first image data, and wherein matching the color information comprises matching the averaged color information for the first image data to corresponding color information from the second image data.
5. The method of claim 1 , wherein the digital image information for each of the exposed test strip and the reference color chart is captured in a single digital image.
6. The method of claim 1 , wherein the digital image information for each of the exposed test strip and the reference color chart is captured under the same ambient photographing conditions, but at different times.
7. The method of claim 1 , wherein matching further comprises matching, by the diagnostic software application executing on the electronic device, color information from the first image data to corresponding color information from the second image data by performing Lab color space analysis on the color information from the first and second image data.
8. The method of claim 1 , wherein the electronic device is a mobile smartphone-type device.
9. The method of claim 1 , wherein the plurality of color blocks comprising the reference color chart are arranged in a plurality of test-specific sequences.