Compositions and Methods for the Therapy and Diagnosis of Influenza
The present invention provides novel human anti-influenza antibodies and related compositions and methods. These antibodies are used in the diagnosis and treatment of influenza infection.
1 - 41 . (canceled)
42 . A recombinant anti-matrix 2 ectodomain (M2e) antibody comprising a variable heavy chain region encoded by a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 43 and a variable light chain region encoded by a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 45.
43 . The antibody of claim 42 , wherein the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 43 is a cDNA and the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 45 is a cDNA.
44 . The antibody of claim 42 , wherein the nucleic acid sequence of SEQ ID NO: 43 is a cDNA and the nucleic acid sequence of SEQ ID NO: 45 is a cDNA.
45 . A vector comprising a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 43 and a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 45.
46 . The vector of claim 45 , wherein the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 43 is a cDNA or the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 45 is a cDNA.
47 . The vector of claim 45 , wherein the vector is vector suitable for propagation or replication of the nucleic acid.
48 . The vector of claim 45 , wherein the vector is vector suitable for expressing the nucleic acid.
49 . The vector of claim 47 or 48 , wherein the vector comprises a virus, a plasmid, or a cosmid.
50 . The vector of claim 49 , wherein the virus is a recombinant virus.
51 . The vector of claim 50 , wherein the virus comprises a promoter obtained from a mammalian genome.
52 . The vector of claim 51 , wherein the mammalian genome is a human genome.
53 . The vector of claim 50 , wherein the virus comprises a promoter obtained from a genome of a virus or a retrovirus.
54 . The vector of claim 53 , wherein the virus or retrovirus is a polyoma virus, fowlpox virus, adenovirus, bovine papilloma virus, avian sarcoma virus, cytomegalovirus (CMV), hepatitis-B virus, or a Simian Virus 40 (SV40).
55 . A recombinant anti-matrix 2 ectodomain (M2e) antibody comprising a nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 44 and a nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 46.
56 . The antibody of claim 55 , wherein the nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 44 is a cDNA and the nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 46 is a cDNA.
57 . A vector comprising a nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 44 and a nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 46.
58 . The vector of claim 57 , wherein the nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 44 is a cDNA or the nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 46 is a cDNA.
59 . The vector of claim 57 , wherein the vector is vector suitable for propagation or replication of the nucleic acid.
60 . The vector of claim 57 , wherein the vector is vector suitable for expressing the nucleic acid.
61 . The vector of claim 59 or 60 , wherein the vector comprises a virus, a plasmid, or a cosmid.
62 . The vector of claim 61 , wherein the virus is a recombinant virus.
63 . The vector of claim 62 , wherein the virus comprises a promoter obtained from a mammalian genome.
64 . The vector of claim 63 , wherein the mammalian genome is a human genome.
65 . The vector of claim 62 , wherein the virus comprises a promoter obtained from a genome of a virus or a retrovirus.
66 . The vector of claim 65 , wherein the virus or retrovirus is a polyoma virus, fowlpox virus, adenovirus, bovine papilloma virus, avian sarcoma virus, cytomegalovirus (CMV), hepatitis-B virus, or a Simian Virus 40 (SV40).
67 . A recombinant anti-matrix 2 ectodomain (M2e) antibody comprising a variable heavy chain region encoded by a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 49 and a variable light chain region encoded by a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 51.
68 . The antibody of claim 67 , wherein the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 49 is a cDNA and the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 51 is a cDNA.
69 . The antibody of claim 67 , wherein the nucleic acid sequence of SEQ ID NO: 49 is a cDNA and the nucleic acid sequence of SEQ ID NO: 51 is a cDNA.
70 . A vector comprising a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 49 and a nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 51.
71 . The vector of claim 70 , wherein the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 49 is a cDNA or the nucleic acid sequence comprising the nucleic acid sequence of SEQ ID NO: 51 is a cDNA.
72 . The vector of claim 70 , wherein the vector is vector suitable for propagation or replication of the nucleic acid.
73 . The vector of claim 70 , wherein the vector is vector suitable for expressing the nucleic acid.
74 . The vector of claim 72 or 73 , wherein the vector comprises a virus, a plasmid, or a cosmid.
75 . The vector of claim 74 , wherein the virus is a recombinant virus.
76 . The vector of claim 75 , wherein the virus comprises a promoter obtained from a mammalian genome.
77 . The vector of claim 76 , wherein the mammalian genome is a human genome.
78 . The vector of claim 75 , wherein the virus comprises a promoter obtained from a genome of a virus or a retrovirus.
79 . The vector of claim 78 , wherein the virus or retrovirus is a polyoma virus, fowlpox virus, adenovirus, bovine papilloma virus, avian sarcoma virus, cytomegalovirus (CMV), hepatitis-B virus, or a Simian Virus 40 (SV40).
80 . A recombinant anti-matrix 2 ectodomain (M2e) antibody comprising a nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 50 and a nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 52.
81 . The antibody of claim 80 , wherein the nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 50 is a cDNA and the nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 52 is a cDNA.
82 . A vector comprising a nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 50 and a nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 52.
83 . The vector of claim 82 , wherein the nucleic acid sequence encoding a variable heavy chain region comprising the amino acid sequence of SEQ ID NO: 50 is a cDNA or the nucleic acid sequence encoding a variable light chain region comprising the amino acid sequence of SEQ ID NO: 52 is a cDNA.
84 . The vector of claim 82 , wherein the vector is vector suitable for propagation or replication of the nucleic acid.
85 . The vector of claim 82 , wherein the vector is vector suitable for expressing the nucleic acid.
86 . The vector of claim 84 or 85 , wherein the vector comprises a virus, a plasmid, or a cosmid.
87 . The vector of claim 86 , wherein the virus is a recombinant virus.
88 . The vector of claim 87 , wherein the virus comprises a promoter obtained from a mammalian genome.
89 . The vector of claim 88 , wherein the mammalian genome is a human genome.
90 . The vector of claim 87 , wherein the virus comprises a promoter obtained from a genome of a virus or a retrovirus.
91 . The vector of claim 90 , wherein the virus or retrovirus is a polyoma virus, fowlpox virus, adenovirus, bovine papilloma virus, avian sarcoma virus, cytomegalovirus (CMV), hepatitis-B virus, or a Simian Virus 40 (SV40).