Polypeptides having glucoamylase activity and polynucleotides encoding same
The present invention relates to polypeptides having glucoamylase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A method of producing a polypeptide having glucoamylase activity, having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18, comprising:
(a) cultivating a host cell transformed with a nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
2. The process of claim 1 , wherein the polypeptide has at least 97% sequence identity to the mature polypeptide of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18.
3. The process of claim 1 , wherein the polypeptide comprises SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 18.
4. A method of producing the polypeptide having glucoamylase activity, having a catalytic domain that has at least 95% sequence identity to the sequence of amino acids 19 to 474 of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14 or amino acids 19 to 471 of SEQ ID NO: 16 or SEQ ID NO: 18, comprising:
(a) cultivating a host cell transformed with a nucleic acid construct comprising a nucleotide sequence encoding the polypeptide under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
5. The process of claim 4 , wherein the polypeptide has at least 95% sequence identity to the sequence of amino acids 18 to 573 of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or amino adds 18 to 576 of SEQ ID NO: 16, or SEQ ID NO: 18.
6. The process of claim 4 , wherein the polypeptide has at least 97% sequence identity to the sequence of amino acids 18 to 573 of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or amino acids 18 to 576 of SEQ ID NO: 16, or SEQ ID NO: 18.