Method of preparing carboxylic acid functionalized polymers
View Patent ↗Methods for preparing water soluble, non-peptidic polymers carrying carboxyl functional groups, particularly carboxylic acid functionalized poly(ethylene glycol) (PEG) polymers are disclosed, as are the products of these methods. In general, an ester reagent R(C═O)OR′, wherein R′ is a tertiary group and R comprises a functional group X, is reacted with a water soluble, non-peptidic polymer POLY-Y, where Y is a functional group which reacts with X to form a covalent bond, to form a tertiary ester of the polymer, which is then treated with a strong base in aqueous solution, to form a carboxylate salt of the polymer. Typically, this carboxylate salt is then treated with an inorganic acid in aqueous solution, to convert the carboxylate salt to a carboxylic acid, thereby forming a carboxylic acid functionalized polymer.
1. A method for preparing a composition of carboxylic acid-functionalized polymers, the method comprising:
i) reacting an ester reagent of the following structure,
wherein X is sulfonate ester,
with a mPEG-OH to form a tertiary ester of the mPEG-OH of the following structure,
ii) treating the tertiary ester of the mPEG-OH with a strong base in aqueous solution to form a carboxylate salt; and
iii) treating the carboxylate salt of the polymer with an inorganic acid in aqueous solution to convert the carboxylate salt to a carboxylic acid, thereby forming a carboxylic acid functionalized polymer of the following structure,
wherein the method results in a composition containing less than 5% by weight of mPEG-OH, with the balance consisting essentially of said carboxylic acid functionalized polymer.
2. The method of claim 1 , wherein the composition contains less than 2% by weight of mPEG-OH.
3. The method of claim 1 , wherein the composition contains less than 0.5% by weight of mPEG-OH.
4. The method of claim 1 , wherein the composition contains substantially no amount of trifluoroacetic acid.
5. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 100 Da to about 100,000 Da.
6. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 200 Da to about 60,000 Da.
7. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 300 Da to about 40,000 Da.
8. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 5,000 Da.
9. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 10,000 Da.
10. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 20,000 Da.
11. The method of claim 1 , wherein the mPEG-OH has a molecular weight of about 40,000 Da.