Biocomposites and methods of making the same
View Patent ↗In general, the present invention is related to biopolymer and biocomposite materials and structures, and methods of making and using the same. In some embodiments, the present invention is directed to oriented collagen based biocomposite materials and structures, and methods of making.
1. A method of producing a fibrillar biopolymer material comprising the steps of:
dipping a thin biopolymer ribbon into a solution, the thin biopolymer ribbon having oriented crimped fibrils; and
pulling the biopolymer ribbon along the long axis of ribbon from the solution in the direction of the crimped biopolymeric fibrils orientation such that the ribbon collapses and folds about the long axis of the ribbon at the air-liquid interface as the ribbon is pulled from the solution to form a fibrillar biopolymer pseudo-fiber wherein the biopolymeric fibrils are aligned along the pseudo-fiber.
2. The method according to claim 1 further comprising drying the fibrillar biopolymer pseudo-fiber.
3. The method according to claim 1 further comprising cross-linking the fibrillar biopolymer pseudo-fiber.
4. The method according to claim 1 wherein the solution has a neutral pH.
5. The method according to claim 1 wherein the solution has a pH in the range of 2 to 9.
6. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber or plurality of pseudo-fibers is further used to deliver cells and components of platelet rich plasma for tissue repair.
7. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is further used to deliver peptides, drugs, growth factors, and small molecules.
8. The method according to claim 1 further comprising: forming a biocomposite structure comprising at least one of the fibrillar biopolymer pseudo-fiber and a biodegradable biocompatible matrix.
9. The method according to claim 1 further comprising: forming a biocomposite structure comprising a plurality of the fibrillar biopolymer pseudo-fibers with arbitrary orientations and a biodegradable biocompatible matrix; and bonding together said plurality of fibrillar biopolymer materials.
10. The method according to claim 8 wherein the biocompatible matrix further comprising at least one of glycosaminoglycans, proteoglycans, vanadate, calcium phosphates, live cells, growth-factors, and their combinations.
11. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is formed for use in cell and tissue culture applications.
12. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is formed for use as an in vivo cell guiding scaffold.
13. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is formed to deliver components of platelet rich plasma.
14. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is formed to deliver live cells for tissue repair.
15. A method of producing a fibrillar biopolymer material comprising the steps of:
dipping a thin biopolymer ribbon into a solution, the thin biopolymer ribbon having oriented crimped fibrils; and
pulling the biopolymer ribbon along the long axis of ribbon from the solution in the direction of the crimped biopolymeric fibrils orientation such that the ribbon collapses and folds about the long axis of the ribbon at the air-liquid interface as the ribbon is pulled from the solution to form a fibrillar biopolymer pseudo-fiber, wherein the solution further comprises nanofibrillar collagen foam which is at least partially incorporated into the formed fibrillar biopolymer pseudo-fiber.
16. A method of producing a fibrillar biopolymer material comprising the steps of:
dipping a thin biopolymer ribbon into a solution, the thin biopolymer ribbon having oriented crimped fibrils; and
pulling the biopolymer ribbon along the long axis of ribbon from the solution in the direction of the crimped biopolymeric fibrils orientation such that the ribbon collapses and folds about the long axis of the ribbon at the air-liquid interface as the ribbon is pulled from the solution to form a fibrillar biopolymer pseudo-fiber, wherein the solution further comprises a dispersion of non-soluble micro-particles which are at least partially incorporated into the formed fibrillar biopolymer pseudo-fiber.
17. The method according to claim 16 wherein the micro-particles have porous structure comprising collagen.
18. The method according to claim 1 wherein the fibrils aligned along the pseudo-fiber are collagen and augment a porous core.
19. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is further freeze-dried.
20. The method according to claim 1 wherein the fibrillar biopolymer pseudo-fiber is used for directional regeneration of lymphatic and blood vessels after intradermal or subcutaneous implantation into a subject.