Porous polymeric formulation prepared using porogens
View Patent ↗An analyte sensor for the continuous or semi-continuous monitoring of physiological parameters and a method for making the analyte sensor are disclosed. The analyte sensor includes a crosslinked copolymer network in contact with a surface of an electrode. The copolymer network has voids formed by the removal of a porogen, and an analyte sensing component is immobilized within the network. The method involves forming a solution of the precursors of the copolymer, depositing the mixture on a surface of an electrode, and curing the deposited mixture to provide the analyte sensor.
1. An analyte sensor comprising:
a crosslinked copolymer network in contact with a surface of an electrode, wherein the crosslinked copolymer network consists of:
backbone chains comprising first methacrylate-derived units and second methacrylate-derived units, wherein each first methacrylate-derived unit has a side chain; and
crosslinks between the second methacrylate-derived units in different backbone chains, wherein the crosslinks comprise poly(alkylene oxide);
an analyte sensing component embedded within the crosslinked copolymer network; and
voids within and defined by the crosslinked copolymer network, wherein the voids are large enough to allow an analyte to pass through the crosslinked copolymer network and small enough to not allow the analyte sensing component to pass through the crosslinked copolymer network.
2. The sensor according to claim 1 , wherein the side chain of the first methacrylate-derived units comprise one or more hydroxy groups.
3. The sensor according to claim 1 , wherein the first methacrylate-derived units have the structure of formula (I):
wherein
X is —O—, —NR′— or —S—;
y is 0-10; and
R 1 is hydrogen, —C 1 -C 12 alkyl, —C 1 -C 12 alkyl-OH, —SiR′ 3 , —C(O)—C 1 -C 12 alkyl, —C 1 -C 12 alkyl-C(O)OR′, wherein R′ is —C 1 -C 12 alkyl.
4. The sensor according to claim 1 , wherein the first methacrylate-derived units have the structure:
5. The sensor according to claim 1 , wherein the crosslinks have the structure of formula (IIa):
wherein w is 2-10.
6. The sensor according to claim 1 , wherein the crosslinks have the structure of formula (IIa):
wherein w is an average value of from about 2 to about 250.
7. The sensor according to claim 1 , wherein the crosslinks are derived from the di(ethylene glycol) portion of di(ethylene glycol) dimethacrylate.
8. The sensor according to claim 1 , wherein the analyte sensing component comprises glucose oxidase.
9. The sensor according to claim 1 , wherein the crosslinked copolymer network has a thickness of about 10 μm to about 30 μm.
10. The sensor according to claim 1 , wherein the first methacrylate-derived units have the structure of formula (I):
wherein
X is —O—, —NR′— or —S—;
y is 0-10; and
R 1 is hydrogen, —C 1 -C 12 alkyl, —C 1 -C 12 alkyl-OH, —SiR′ 3 , —C(O)—C 1 -C 12 alkyl, —C 1 -C 12 alkyl-C(O)OR′, wherein R′ is —C 1 -C 12 alkyl;
the crosslinks have the structure of formula (IIa):
wherein w is 2-10; and
the analyte sensing component comprises glucose oxidase.
11. The sensor of claim 8 , wherein the analyte is glucose and the voids within and defined by the crosslinked copolymer network are large enough to allow glucose to pass through the crosslinked copolymer network and small enough to not allow glucose oxidase to pass through the crosslinked copolymer network.