Topical Formulations Comprising DNA Repair Enzymes, and Methods of Use Thereof
Disclosed are methods of decreasing or preventing UV-induced skin damage, comprising the step of applying to an area of skin an effective amount of a topical formulation comprising a photolyase and an endonuclease. In certain embodiments, the formulation is applied before and after UV exposure.
1 . A topical formulation, comprising a photolyase; an endonuclease; and a dermatologically acceptable carrier or excipient.
2 . The topical formulation of claim 1 , wherein the photolyase is from A. nidulans.
3 . The topical formulation of claim 1 , wherein the endonuclease is from M. luteus.
4 . The topical formulation of claim 1 , wherein the photolyase is from A. nidulans ; and the endonuclease is from M. luteus.
5 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
a diluent
from about 35 to about 95
a humectant or a solvent
from about 5 to about 15
a skin conditioning agent or an emollient
from about 4 to about 12
a DNA repair enzyme
from about 1.5 to about 4.5
a suspending agent
from about 0.1 to about 2
an emulsion stabilizer, an emulsifier, or
from about 2.5 to about 7.5
a surfactant
an antioxidant
from about 0.5 to about 1.5
a chelating agent
from about 0.05 to about 0.15
a preservative
from about 0.4 to about 1.2
a pH adjuster
6 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
Water
from about 35 to about 95
Glycerin
from about 3 to about 11
Ethylhexyl Palmitate
from about 2 to about 6
Cetearyl Alcohol
from about 1 to about 3
Propylene Glycol
from about 1 to about 4
Dicetyl Phosphate
from about 0.5 to about 2.5
Theobroma Grandiflorum Seed Butter
from about 1 to about 3
Petrolatum
from about 0.5 to about 1.5
Dimethicone
from about 0.5 to about 1.5
A. nidulans Extract
from about 0.5 to about 1.5
Arabidopsis Thaliana Extract
from about 0.5 to about 1.5
Micrococcus Luteus Lysate
from about 0.5 to about 1.5
Lecithin
from about 0.5 to about 1.5
Ceteareth-10 Phosphate
from about 0.3 to about 0.9
Steareth-10
from about 0.3 to about 0.9
Hydroxypropyl Bispalmitamide MEA
from about 0.2 to about 0.8
(Ceramide)
Tocopheryl Acetate
from about 0.5 to about 1.5
Disodium EDTA
from about 0.05 to about 0.15
Phenoxyethanol
from about 0.4 to about 1.2
Sodium Hyaluronate
from about 0.05 to about 0.15
Sodium Hydroxide
7 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
% by weight of
Ingredient
topical formulation
Water
about 71.64
Glycerin
about 7.50
Ethylhexyl Palmitate
about 4.00
Cetearyl Alcohol
about 2.00
Propylene Glycol
about 2.50
Dicetyl Phosphate
about 1.40
Theobroma Grandiflorum Seed Butter
about 2.00
Petrolatum
about 1.00
Dimethicone
about 1.00
A. nidulans Extract
about 1
Arabidopsis Thaliana Extract
about 1
Micrococcus Luteus Lysate
about 1
Lecithin
about 1
Ceteareth-10 Phosphate
about 0.60
Steareth-10
about 0.86
Hydroxypropyl Bispalmitamide MEA
about 0.50
(Ceramide)
Tocopheryl Acetate
about 1.00
Disodium EDTA
about 0.10
Phenoxyethanol
about 0.80
Sodium Hyaluronate
about 0.10
Sodium Hydroxide
8 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
a diluent
from about 45 to about 95
a thickener
from about 0.25 to about 0.8
a humectant or a solvent
from about 4 to about 12
an anti-irritant
from about 0.5 to about 1.5
a DNA repair enzyme
from about 1.5 to about 4.5
a suspending agent
from about 0.5 to about 1.5
an emollient
from about 3 to about 9
a chelating agent
from about 0.05 to about 0.15
a preservative
from about 0.5 to about 1.5
pH Adjuster
9 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
Water
from about 45 to about 95
Carbomer 980
from about 0.1 to about 0.6
Acrylates/C10-C30 Alkyl Acrylate
from about 0.1 to about 0.3
Crosspolymer
Glycerin
from about 1.5 to about 4.5
Propanediol
from about 1.5 to about 4.5
Disodium EDTA
from about 0.05 to about 0.15
Sodium Hyaluronate
from about 1 to about 3
Evodia Rutaecarpia Fruit Extract
from about 0.5 to about 1.5
A. nidulans Extract
from about 0.5 to about 1.5
Arabidopsis Thaliana Extract
from about 0.5 to about 1.5
Micrococcus Luteus Lysate
from about 0.5 to about 1.5
Lecithin
from about 0.5 to about 1.5
Ethylhexyl Isononanoate
from about 2.50
Isononyl Isononanoate
from about 2.50
Butylene Glycol
from about 0.0001
BHT
from about <1%
Ergothioneine
from about 0.0001
Santalam Album (Sandalwood) Extract
from about 0.33
Phellodendron Amurense Bark Extract
from about 0.33
Hordeum Distichon (Barley) Extract
from about 0.33
Sodium Hydroxide
10 . The topical formulation of claim 1 , wherein the topical formulation consists essentially of
%, by weight of the
Ingredient
topical formulation
Water
about 85.85
Carbomer 980
about 0.35
Acrylates/C10-C30 Alkyl Acrylate
about 0.20
Crosspolymer
Glycerin
about 3.00
Propanediol
about 3.00
Disodium EDTA
about 0.10
Sodium Hyaluronate
about 2.00
Evodia Rutaecarpia Fruit Extract
about 1
A. nidulans Extract
about 1
Arabidopsis Thaliana Extract
about 1
Micrococcus Luteus Lysate
about 1
Lecithin
about 1
Ethylhexyl Isononanoate
about 2.50
Isononyl Isononanoate
about 2.50
Butylene Glycol
about 0.0001
Butylated hydroxy toluene (BHT)
about 1
Ergothioneine
about 0.0001
Santalam Album (Sandalwood) Extract
about 0.33
Phellodendron Amurense Bark Extract
about 0.33
Hordeum Distichon (Barley) Extract
about 0.33
Sodium Hydroxide
11 . A method of decreasing or preventing UV-induced skin damage, comprising the step of applying to an area of skin an effective amount of a topical formulation comprising a photolyase; an endonuclease; and a dermatologically acceptable carrier or excipient.
12 . The method of claim 11 , wherein the photolyase is from A. nidulans.
13 . The method of claim 11 , wherein the endonuclease is from M. luteus.
14 . The method of claim 11 , wherein the photolyase is from A. nidulans ; and the endonuclease is from M. luteus.
15 . The method of claim 11 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
a diluent
from about 35 to about 95
a humectant or a solvent
from about 5 to about 15
a skin conditioning agent or an emollient
from about 4 to about 12
a DNA repair enzyme
from about 1.5 to about 4.5
a suspending agent
from about 0.1 to about 2
an emulsion stabilizer, an emulsifier, or
from about 2.5 to about 7.5
a surfactant
an antioxidant
from about 0.5 to about 1.5
a chelating agent
from about 0.05 to about 0.15
a preservative
from about 0.4 to about 1.2
a pH adjuster
16 . The method of claim 11 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
Water
from about 35 to about 95
Glycerin
from about 3 to about 11
Ethylhexyl Palmitate
from about 2 to about 6
Cetearyl Alcohol
from about 1 to about 3
Propylene Glycol
from about 1 to about 4
Dicetyl Phosphate
from about 0.5 to about 2.5
Theobroma Grandiflorum Seed Butter
from about 1 to about 3
Petrolatum
from about 0.5 to about 1.5
Dimethicone
from about 0.5 to about 1.5
A. nidulans Extract
from about 0.5 to about 1.5
Arabidopsis Thaliana Extract
from about 0.5 to about 1.5
Micrococcus Luteus Lysate
from about 0.5 to about 1.5
Lecithin
from about 0.5 to about 1.5
Ceteareth-10 Phosphate
from about 0.3 to about 0.9
Steareth-10
from about 0.3 to about 0.9
Hydroxypropyl Bispalmitamide MEA
from about 0.2 to about 0.8
(Ceramide)
Tocopheryl Acetate
from about 0.5 to about 1.5
Disodium EDTA
from about 0.05 to about 0.15
Phenoxyethanol
from about 0.4 to about 1.2
Sodium Hyaluronate
from about 0.05 to about 0.15
Sodium Hydroxide
17 . The method of claim 11 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
Water
about 71.64
Glycerin
about 7.50
Ethylhexyl Palmitate
about 4.00
Cetearyl Alcohol
about 2.00
Propylene Glycol
about 2.50
Dicetyl Phosphate
about 1.40
Theobroma Grandiflorum Seed Butter
about 2.00
Petrolatum
about 1.00
Dimethicone
about 1.00
A. nidulans Extract
about 1
Arabidopsis Thaliana Extract
about 1
Micrococcus Luteus Lysate
about 1
Lecithin
about 1
Ceteareth-10 Phosphate
about 0.60
Steareth-10
about 0.86
Hydroxypropyl Bispalmitamide MEA
about 0.50
(Ceramide)
Tocopheryl Acetate
about 1.00
Disodium EDTA
about 0.10
Phenoxyethanol
about 0.80
Sodium Hyaluronate
about 0.10
Sodium Hydroxide
18 . The method of claim 11 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
a diluent
from about 45 to about 95
a thickener
from about 0.25 to about 0.8
a humectant or a solvent
from about 4 to about 12
an anti-irritant
from about 0.5 to about 1.5
a DNA repair enzyme
from about 1.5 to about 4.5
a suspending agent
from about 0.5 to about 1.5
an emollient
from about 3 to about 9
a chelating agent
from about 0.05 to about 0.15
a preservative
from about 0.5 to about 1.5
pH Adjuster
19 . The method of claim 11 , wherein the topical formulation consists essentially of
% by weight of the
Ingredient
topical formulation
Water
from about 45 to about 95
Carbomer 980
from about 0.1 to about 0.6
Acrylates/C10-C30 Alkyl Acrylate
from about 0.1 to about 0.3
Crosspolymer
Glycerin
from about 1.5 to about 4.5
Propanediol
from about 1.5 to about 4.5
Disodium EDTA
from about 0.05 to about 0.15
Sodium Hyaluronate
from about 1 to about 3
Evodia Rutaecarpia Fruit Extract
from about 0.5 to about 1.5
A. nidulans Extract
from about 0.5 to about 1.5
Arabidopsis Thaliana Extract
from about 0.5 to about 1.5
Micrococcus Luteus Lysate
from about 0.5 to about 1.5
Lecithin
from about 0.5 to about 1.5
Ethylhexyl Isononanoate
from about 2.50
Isononyl Isononanoate
from about 2.50
Butylene Glycol
from about 0.0001
BHT
from about <1%
Ergothioneine
from about 0.0001
Santalam Album (Sandalwood) Extract
from about 0.33
Phellodendron Amurense Bark Extract
from about 0.33
Hordeum Distichon (Barley) Extract
from about 0.33
Sodium Hydroxide
20 . The method of claim 11 , wherein the topical formulation consists essentially of
%, by weight of the
Ingredient
topical formulation
Water
about 85.85
Carbomer 980
about 0.35
Acrylates/C10-C30 Alkyl Acrylate
about 0.20
Crosspolymer
Glycerin
about 3.00
Propanediol
about 3.00
Disodium EDTA
about 0.10
Sodium Hyaluronate
about 2.00
Evodia Rutaecarpia Fruit Extract
about 1
A. nidulans Extract
about 1
Arabidopsis Thaliana Extract
about 1
Micrococcus Luteus Lysate
about 1
Lecithin
about 1
Ethylhexyl Isononanoate
about 2.50
Isononyl Isononanoate
about 2.50
Butylene Glycol
about 0.0001
Butylated hydroxy toluene (BHT)
about 1
Ergothioneine
about 0.0001
Santalam Album (Sandalwood) Extract
about 0.33
Phellodendron Amurense Bark Extract
about 0.33
Hordeum Distichon (Barley) Extract
about 0.33
Sodium Hydroxide