DETECTION OF TARGET ANALYTES USING PARTICLES AND ELECTRODES
The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.
1 - 6 . (canceled)
7 . A method of detecting the presence of a target analyte comprising:
a. providing an electrode comprising:
i. a covalently attached a first capture binding ligand;
ii. a colloidal particle comprising a second capture binding ligand; and
iii. a target analyte, bound to said first and said second ligands; and
b. detecting an electronic signal from said electrode as an indication of the presence of said target analyte.
8 . A method according to claim 7 wherein said first and said second capture binding ligands are nucleic acid sequences and said target analyte is a nucleic acid sequence.
9 . A method according to claim 7 or 8 wherein said colloidal particle comprises a material selected from the group consisting of Au, Se, Te, Co, Ni, Fe, Cu and Pt.
10 . A method according to claim 9 wherein said colloidal particle comprises Au.
11 . A method according to claim 7 wherein said detecting utilizes AC voltametry.
12 . A method of detecting the presence of a target analyte comprising:
a. providing an array of electrodes, each electrode comprising:
i. a covalently attached first nucleic acid probe;
ii. a colloidal particle comprising a second nucleic acid probe; and
iii. a target nucleic acid, hybridized to said first and said second nucleic acid probes;
b. detecting a signal from said electrode as an indication of the presence of said target analyte.
13 . A method according to claim 7 wherein said signal is electrical.
14 . A method according to claim 7 wherein said signal is optical.
15 . A method according to claim 12 wherein said colloidal particle comprises Au.
16 . A method of detecting a target analyte comprising forming an assay complex comprising contacting:
a. a magnetic particle comprising a first capture binding ligand;
b. a colloidal particle comprising:
i) a second capture binding ligand; and
ii) a third binding ligand different from said second binding ligand
c. a target analyte, bound to said first and said second binding ligands.
17 . A method according to claim 8 wherein said colloidal particle comprises a material selected from the group consisting of Au, Se, Te, Co, Ni, Fe, Cu and Pt.